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Mouse Tail SuperDirect PCR Kit
Wannan kayan aikin yana amfani da tsarin kariya na lysis na musamman don sakin DNA na jini daga wutsiyar linzamin kwamfuta da samfuran kunnen linzamin kwamfuta na PCR, don haka ya dace musamman don gwajin sikelin halittu.
An saki aikin sakin DNA daga kwayoyin layin a tsakanin minti 10-30 a zazzabin ɗaki (20-25 ° C). Babu buƙatar dumama, kuma sauran matakai kamar furotin da cire RNA ba'a buƙata. Ana iya amfani da adadin DNA da aka saki azaman samfuri don aikin PCR.
2, M-PCR Easy TM Mix yana da juriya mai ƙarfi ga masu hanawa na PCR, kuma zai iya amfani da lysate ɗin samfurin don a gwada shi azaman samfuri don ingantaccen da takamaiman faɗaɗawa. Wannan reagent din ya kunshi Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, ajiyar amsawa, mai inganta PCR da karfafawa.
D-Taq DNA polymerase shine polymerase na DNA wanda aka haɓaka ta musamman ta Foregene don halayen PCR kai tsaye. D-Taq DNA polymerase yana da juriya mai ƙarfi ga nau'o'in masu hana PCR, kuma zai iya inganta haɓakar DNA sosai a cikin tsarin rikice-rikice masu rikitarwa, kuma saurin haɓaka zai iya isa 2Kb / min, wanda ya dace musamman don aikin PCR kai tsaye.
