-
Mouse Tail Direct PCR Kit
Wannan kayan aikin yana amfani da tsarin kariya na lysis na musamman don saurin sakin DNA daga wutsiyar linzamin kwamfuta, kunnuwa, tsokoki da sauran samfuran nama don halayen PCR, saboda haka ya dace musamman don gwajin kwayar halitta mai girma.
Hanyar sakewa da kwayoyin halittar Halitta (DNA) daga buhunan lysis an kammala shi tsakanin minti 10-30 a 65 ° C. Babu wasu matakai kamar furotin da cire RNA da ake buƙata, kuma ana iya amfani da DNA ɗin da aka sake a matsayin samfuri don aikin PCR.
2 × M1-PCR Easy TM Mix yana da juriya mai ƙarfi ga masu hanawa na PCR, kuma zai iya amfani da lysate ɗin samfurin don a gwada shi azaman samfuri don ingantaccen da takamaiman faɗaɗawa. Wannan reagent din ya kunshi Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, ajiyar amsawa, mai inganta PCR da karfafawa.
D-Taq DNA polymerase shine polymerase na DNA wanda aka haɓaka ta musamman ta Foregene don halayen PCR kai tsaye. D-Taq DNA polymerase yana da juriya mai ƙarfi ga nau'o'in masu hana PCR, kuma zai iya inganta haɓakar DNA sosai a cikin tsarin rikice-rikice masu rikitarwa, kuma saurin haɓaka zai iya isa 2Kb / min, wanda ya dace musamman don aikin PCR kai tsaye.
