RT-qPCR Easyᵀᴹ (Mataki ɗaya) -SYBR Green I
Bayanin Kit:
◮Kit ɗin mataki ɗaya yana yin jujjuya rubutu da qPCR halayen biyu a cikin bututu ɗaya, kawai buƙatar ƙara samfuri RNA, takamaiman PCR da ddH na kyauta na RNase.2O.
◮Kit ɗin na iya yin nazarin RNA cikin sauri da inganci cikin ƙididdigewa ko kuma gano RNA.
◮Kit ɗin yana amfani da na musamman na Foregene reverse reagent reagent da Foregene HotStar Taq DNA Polymerase haɗe tare da keɓaɓɓen tsarin amsawa don inganta ingantaccen haɓakawa da ƙayyadaddun halayen.
◮Ingantattun tsarin amsawa yana sa abin ya sami mafi girman ganewar ganewa, ingantaccen yanayin zafi, da mafi kyawun haƙuri.
◮RT-qPCR Mai SauƙiTM(Mataki Daya) -SYBR Green I kit ya zo tare da rini na ciki na ROX, wanda za'a iya amfani dashi don kawar da bayanan sigina da kurakuran sigina tsakanin rijiyoyi, wanda ya dace da abokan ciniki don amfani da su a cikin nau'ikan kayan aikin PCR masu yawa.
Bayani
RT-qPCR Mai SauƙiTM (Mataki Daya) -SYBR Green I yana amfani da wani tsari na musamman don haɗa halayen RT da Real Time PCR yadda ya kamata, wanda zai iya hanzarta kammala gano adadin kwayoyin halitta.Samfurin yana da juriya mai ƙarfi, ƙayyadaddun ƙayyadaddun ƙima da kwanciyar hankali.Samfurin ya ƙunshi na musamman na kamfanin Foregene HotStar Taq DNA Polymerase, wanda ke da fa'idodin haɓakawa da sauri (2Kb/min), ingantaccen haɓakawa, ƙaƙƙarfan ƙayyadaddun ƙarfin haɓakawa, da ƙarancin daidaitawa idan aka kwatanta da talakawa Taq enzymes.Ana amfani da shi anan don Real Time RT-PCR don rage ƙayyadaddun haɓakawa da haɓaka daidaiton PCR.
Ƙayyadaddun bayanai
100T (20μl tsarin)
500T (20μl tsarin)
Abubuwan Kit
| RT-qPCR Mai SauƙiTM(Mataki Daya) -SYBR Green I | ||
| Abubuwan da ke cikin Kit | RT-02111 | RT-02112 |
| 100T (20μl tsarin) | 500T (20μl tsarin) | |
| 2 × RT-qPCR Mai SauƙiTM Mix-SYBR | 1 ml | 1.7ml × 3 |
| 50× ROX Reference Dye | 200 μl | 1 ml |
| RNase-Free ddH2O | 1.7ml ku | 1.7ml × 3 |
| Jagoran Jagora | 1 guda | guda 1 |
Fasaloli & fa'idodi
- Kit ɗin mataki ɗaya yana yin jujjuya rubutu da qPCR halayen biyu a cikin bututu ɗaya, kawai buƙatar ƙara samfuri RNA, ƙayyadaddun abubuwan PCR da RNase-Free ddH2O.
- Kit ɗin na iya yin nazarin RNA cikin sauri da inganci cikin ƙima ko kuma gano RNA.
- Kit ɗin yana amfani da na musamman na Foregene reverse reagent reagent da Foregene HotStar Taq DNA Polymerase haɗe tare da keɓaɓɓen tsarin amsawa don haɓaka ingantaccen haɓakawa da ƙayyadaddun halayen.
- Ingantaccen tsarin amsawa yana sa abin ya sami mafi girman ganewar ganewa, ingantaccen yanayin zafi, da mafi kyawun haƙuri.
- RT-qPCR Mai SauƙiTM(Mataki Daya) -SYBR Green I kit ya zo tare da rini na ciki na ROX, wanda za'a iya amfani dashi don kawar da bayanan sigina da kurakuran sigina tsakanin rijiyoyi, wanda ya dace da abokan ciniki don amfani da su a cikin nau'ikan kayan aikin PCR masu yawa.
Gudun aiki
Yanayin ajiya
1.Sufuriyanayi
Dukkanin tsarin sufurin akwatin kankara mai ƙarancin zafin jiki, don tabbatar da cewa kit ɗin yana cikin yanayin <4 ℃.
2.Adana yanayi
Ana adana kit ɗin a -20 ℃.Ajiye samfurin a cikin firjin zafin jiki akai-akai a-20 ℃ nan da nan bayan samu.Idan yanayin ajiya ya dace, samfurinba zai lalata kowane aiki a cikin lokacin ingancin shekara 1 ba.
