Kit ɗin ForeDirect RT-qPCR
Bayanin Kit:
◮Don haɓakawa na RNA kai tsaye daga tarin swab ba tare da hanyoyin tsarkakewa na RNA ba.Wannan kit ɗin yana kammala zagayowar qRT-PCR a cikin awa ɗaya.Haɗin enzyme shine ingantaccen haɗakar Reverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor.Reaction Buffer ya ƙunshi duk abubuwan da ake buƙata, gami da ingantattun abubuwan buffer, Mg2+, dUTP, da dNTPs.
Bayani
Kit ɗin ForeDirect RT-qPCR an ƙirƙira shi don haɓakawa ta RNA kai tsaye daga tarin swab ba tare da aiwatar da tsarkakewar RNA ba.Wannan kit ɗin yana kammala zagayowar qRT-PCR a cikin awa ɗaya.Haɗin enzyme shine ingantaccen haɗakar Reverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor.Reaction Buffer ya ƙunshi duk abubuwan da ake buƙata, gami da ingantattun abubuwan buffer, Mg2+, dUTP, da dNTPs.
Ƙayyadaddun bayanai
100T
Abubuwan Kit
| Nucleic acid wakili saki |
| Mai kare RNA |
| 2 × RT-qPCR Buffer |
| Cakudar Enzyme (Taq&M-MLV) |
| Umarni |
PCR Application
1. Da zarar PCR dauki bututu an takaicce centrifuged, sanya su a cikin samfurin tanki na ƙarawa kayan aiki.
2. Thermal yarjejeniya
| Mataki | Zazzabi | Lokaci | Zagaye | |
| 1 | Juya rubutun | 50 ℃ | 15 min | 1 |
| 2 | Pre-denaturation | 95 ℃ | 1 min | 1 |
| 3 | Denaturation | 95 ℃ | 10 seconds | 42 |
| 4 | Anneal/Extension | 60 ℃ | 30 seconds | |
Lura:An gano siginar mai walƙiya nan da nan bayan matakin tsawo na kowane zagaye.
3. Bayan saitin, ajiye fayil ɗin kuma gudanar da shirin amsawa.
Matakan Aiki
Kafin shirye-shiryen reagent, duk reagents a cikin kit ɗin yakamata a narke a zafin jiki kuma a gauraye su a hankali.
Reagent shiri
1. Shirya Haɗin Sakin Nucleic Acid
| Bangaren | Ƙarar kowane Samfura ko Sarrafa |
| Nucleic acid wakili saki | 5 μl |
| Mai kare RNA | 0.5 μl |
2. Shirya Mix Reaction
| Bangaren | Ƙarar kowane Samfura ko Sarrafa |
| 2 × RT-qPCR Buffer | 15 μl |
| Cakudar Enzyme (Taq&M-MLV) | 1.5 μl |
| Firimiya da Bincike | 1 μl |
Adana da Rayuwar Shelf
-Ya kamata a adana kayan a -20± 5℃.Lokacin tabbatarwa shine watanni 12.
-A guji maimaita daskare-zagaye (<5 cycles).
