Kit ɗin RT-qPCR kai tsaye
Bayanin Kit:
◮ Kit ɗin RT-qPCR kai tsaye, ta amfani da Foreasy Reverse Transcriptase da Foreasy HS Taq DNA Polymerase haɓaka ta Foregene, tare da keɓancewar amsawa, yana sanya wannan kit ɗin tare da juriya mai ƙarfi da dacewa, kuma yana iya gwada amsa kai tsaye ta amfani da tsarin Foregene Lysis azaman samfuri.Wannan kit ɗin na iya nema don COVID-19 kayan gano nucleic acid da sauran abubuwan haɓaka kayan gano ƙwayoyin ƙwayoyin cuta na nucleic acid.
Bayani
Kit ɗin RT-qPCR kai tsaye yana ba da fakitin RT-qPCR daban da ingantaccen enzyme mai ingancicakuda (Taq, M-MLV, RI), yin shi dace don ƙirƙirar baya-karshen kaya da tsarin.daidaitawa, a cikin sauki tabbaci.
Ƙayyadaddun bayanai
500T, 50,000T
Abubuwan Kit
| Abubuwan da ke ciki (20 μL tsarin amsawa) | Saukewa: IM-05111-01 | Saukewa: IM-05112-01 |
| 500 T | 50,000 T | |
| Cakudar Enzyme Kai tsaye | 500 μl×1 | ml 50×1 |
| 2× Direct RT-qPCR Buffer | 1 ml×5 | 500 ml×1 |
| Umarni | 1 | 1 |
Matakan Aiki
Kafin shirye-shiryen reagent, duk reagents a cikin kit ɗin yakamata a narke a zafin jiki kuma a gauraye su a hankali.
A: Shirya of samfuri kuma reagent
1. Shirya samfurin RNA da aka shirya (An ba da shawarar yin amfani da Foregene Total RNA Isolation Kit jerin kit don cirewa da tsarkake samfurin RNA) ko samfurin fatattaka samfurin (An ba da shawarar yin amfani da tsarin Foregene Lysis), ƙayyadaddun ƙayyadaddun abubuwa (10 μM) da sauran abubuwan da suka dace, kayan aiki.
2. Saka 2× Direct RT-qPCR Buffer, RNase-Free ddH2O da 20× ROX Reference Dye (idan ya's buƙata) a cikin akwati tare da ƙanƙara, yana sanya waɗannan narke a hankali, kuma a haɗa su a hankali.
B: Shirya of RT-qPCR tsarin
Tebur 1 : Shirya of RT-qPCR stsarin
| Bangaren | Ƙarar | Natsuwa na ƙarshe |
| 2× Direct RT-qPCR Buffer | 10 μl | 1× |
| Cakudar Enzyme Kai tsaye | 1μL | |
| Gaban Farko (10 μM) | 0.8 ml | 50-900nM |
| Juya Farko (10 μM) | 0.8 ml | 50-900nM |
| Binciken (4 μM) | 1μL | 200nM |
| Samfura (RNA ko Lysate) | X μL | |
| 20 × ROX Magana Rini | - | 1* |
| RNase-FreeddH2O | (6.4-X) μL | |
| Jimlar Ƙarfafa | 20μL |
C: Saita da RT-qPCR dauki shirin
Tebur 2 : RT-qPCR amsawaon shirin saitin (domin misali: biyu-mataki hanya)
| Mataki | Zazzabi | Lokaci | Zagaye | Abun ciki | |
| 1 | 50 ℃ | 15 min | 1* | 1 | Juya rubutun |
| 2 | 95 ℃ | 1 min | 1 | Pre-denaturation | |
| 3 | 95 ℃ | 10 dakika | 40-45 | Denaturation | |
| 60 ℃ | 30 seconds 2* | Anneal/Extension | |||
Adana da Rayuwar Shelf
-Ya kamata a adana kayan a -20± 5℃.Lokacin tabbatarwa shine watanni 12.
-A guji maimaita daskare-zagaye (<5 cycles).
