Kai tsaye RT-qPCR Kit II
Bayanin Kit:
◮Kit ɗin RT-qPCR kai tsaye, ta amfani da Foreasy Reverse Transcriptase da Foreasy HS Taq DNA Polymerase haɓaka ta Foregene, tare da keɓancewar amsawa, yana sanya wannan kit ɗin tare da juriya mai ƙarfi da dacewa, kuma yana iya gwada amsa kai tsaye ta amfani da tsarin Foregene Lysis azaman samfuri.Wannan kit ɗin na iya nema don COVID-19 kayan gano nucleic acid da sauran abubuwan haɓaka kayan gano ƙwayoyin ƙwayoyin cuta na nucleic acid.
Bayani
Kit ɗin RT-qPCR kai tsaye yana ba da fakitin RT-qPCR daban, babban ingantaccen Foreasy HS Taq DNA Polymeraseda Foreasy Reverse Transcriptase (MMLV),yin dacewa don ƙirƙirar kayan aiki na baya-baya da daidaita tsarin, a cikin tabbatarwa mai sauƙi.
Kit ɗin RT-qPCR kai tsaye, ta amfani da Foreasy Reverse Transcriptase da Foreasy HS Taq DNA Polymerase haɓaka ta Foregene, tare da keɓancewar amsawa, yana sanya wannan kit ɗin tare da juriya mai ƙarfi da dacewa, kuma yana iya gwada amsa kai tsaye ta amfani da tsarin Foregene Lysis azaman samfuri.Wannan kit ɗin na iya nema don COVID-19 kayan gano nucleic acid da sauran abubuwan haɓaka kayan gano ƙwayoyin ƙwayoyin cuta na nucleic acid.
Wannan kit ɗin na iya zama ɓangaren samfurin IVD kai tsaye, ta amfani da sauƙi da sauri.Yana buƙatar tabbatarwa mai sauƙi kawai, ba tare da sake haɓakawa ba.
Ƙayyadaddun bayanai
500T, 50,000T
Abubuwan Kit
| Abubuwan da ke ciki (20 μL tsarin amsawa) | Saukewa: IM-05111-02 | Saukewa: IM-05112-02 |
| 500 T | 50,000 T | |
| Farkon RHarshen Transcriptase (200 U/μL) | 50 μl×1 | 1 ml×5 |
| Foreasy HS Taq DNA Polymerase (5 U/μL) | 100 μl×1 | 1 ml×10 |
| 2× Direct RT-qPCRBuffer | 1 ml ×5 | 500 ml × 1 |
| Umarni | 1 | 1 |
Matakan Aiki
A: Shirya samfuri da reagent
1. Shirya samfurin RNA da aka shirya (An ba da shawarar yin amfani da Foregene Total RNA Isolation Kit jerin kit don cirewa da tsarkake samfurin RNA) ko samfurin fatattaka samfurin (An ba da shawarar yin amfani da tsarin Foregene Lysis), ƙayyadaddun ƙayyadaddun abubuwa (10 μM) da sauran abubuwan da suka dace, kayan aiki.
2. Saka 2 × Direct RT-qPCR Buffer, RNase-Free ddH2O da 20 × ROX Reference Dye (idan ana buƙata) a cikin akwati tare da kankara, yin waɗannan narke a hankali, kuma a hade a hankali.
B: Shirya tsarin RT-qPCR
Samu rabin ƙarar ƙarar martani na tsarin amsawa (misali, idan girman tsarin shine 20 μL, yana buƙatar samun 10 μL 2 × Direct RT-qPCR Buffer). Domindaidai adadin enzyme,muna ba da shawarar hakaM-MLV: 10-30 U/20 μL tsarin amsawa,TaqDNA polymerase:1-2 U/20 μL tsarin amsawawannan shine shawararmu, yakamata ku gwada ku daidaita ta), ƙara samfuri na RNA, ƙayyadaddun firamare da bincike, kuma ƙara ddH-Free-RNase2O zuwa 20 μl.Musamman shirye-shiryen tsarin amsawar RT-qPCR ana iya komawa zuwa tebur mai zuwa 1.
Table 1: Shirya tsarin RT-qPCR
| Bangaren | Ƙarar | Natsuwa na ƙarshe |
| 2× Direct RT-qPCR Buffer | 10 μl | 1× |
| Rubuce-rubucen Haɗin Kai (MMLV) | 10-30 U | |
| Foreasy HS Taq DNA Polymerase | 1-2 U | |
| Gaban Farko (10 μM) | 0.8 ml | 50-900nM |
| Juya Farko (10 μM) | 0.8 ml | 50-900nM |
| Binciken (4 μM) | 1 μl | 200nM |
| Samfura (RNA ko Lysate) | X μL | |
| 20× ROX Reference Dye | - | 1* |
| RNase-FreddH2O | (6.4-X) μL | |
| Jimlar Ƙarfafa | 20 μl |
Lura: Gaban Farko da Reverse Primer su ne ƙayyadaddun jigo na jigon jigon manufa.Ana iya daidaita tsarin qPCR bisa ga ingantacciyar gwaji da ƙirar PCR.Muna ba da shawarar 400nM don ƙaddamarwar ƙarshe na mafi yawan abubuwan farko.Daidaita ƙarar ƙayyadaddun firamare da bincike bisa ga shirye-shiryen tattarawa da ƙaddamarwar ƙarshe da aka ba da shawarar, don Allah.
1 *: Zaɓi daidaitaccen taro na ƙarshe na ROX Reference Dye bisa ga kayan aikin PCR daban-daban.Mafi kyawun ROX Reference Dye na kayan aikin PCR na gama gari ana nunawa a cikin tebur mai zuwa:
| Kayan aikin PCR masu ƙima | Ƙarshe na Ƙarshe na ROX Reference Dye |
| ABI PRISM 7000/7300/7700/7900HT/Mataki na daya,da dai sauransu. | 1 × (misali, ƙara 1μl 20 × ROX Reference Dye a cikin tsarin amsawa na 20 μL) |
| ABI 7500, 7500 Fast, Stratagene Mx3000P, Mx3005P da Mx4000, da dai sauransu. | 0.5 × (misali, ƙara 0.5μl 20 × ROX Reference Dye a cikin tsarin amsawa na 20 μL) |
| Roche PCR kayan aiki, Bio-Rad PCR kayan aiki, Eppendorf adadi PCR kayan aiki, da dai sauransu. | Ba tare da ROX Reference Dye ba |
Adana da Rayuwar Shelf
Ya kamata a adana kit ɗin a -20 ± 5 ℃.Ya kamata a adana shi nan da nan a cikin -20 ℃ thermostatic firiji.Lokacin tabbatarwa shine shekaru 2 idan an adana shi cikin yanayin da ya dace.
