RT Easy II (tare da gDNase) Master Premix don haɗin cDNA na farko don PCR na ainihi tare da gDNase
Bayanin Kit:
-Ingantacciyar ikon cire gDNA, wanda zai iya cire gDNA a cikin samfuri a cikin mintuna 2.
-Ingantaccen tsarin jujjuya rubutu, yana ɗaukar mintuna 15 kawai don kammala haɗin layin farko na cDNA.
-Complex samfuri: samfura tare da babban abun ciki na GC da hadaddun tsarin sakandare kuma ana iya juyawa tare da ingantaccen inganci.
-Tsarin jujjuya juzu'i mai girma, samfuran pg-matakin kuma na iya samun cDNA mai inganci.
-Tsarin rubutun baya yana da babban kwanciyar hankali na thermal, mafi kyawun zafin jiki shine 42℃, kuma har yanzu yana da kyakkyawan aikin juzu'i a 50℃.
Ƙayyadaddun bayanai
25×20µl rxns,100×20µl rxns
RT SauƙiTMII (Tare da gDNase) tsarin juzu'i ne wanda aka haɓaka musamman don PCR na Real Time don cire gurɓatar DNA da sauri.5×gDNase Mix zai iya hanzarta cire ragowar kwayoyin halitta a cikin RNA a 42°C na mintuna 2, yadda ya kamata ya guje wa tsoma bakin kwayoyin halitta akan sakamakon qPCR.
5× RO-SaukiTMMix ya ƙunshi Foregene Reverse Transcriptase musamman wanda Foregene ya haɓaka, wanda shine sabon nau'in juzu'i mai ƙarfi tare da kusancin RNA.Ingantacciyar tsarin yana sa jujjuyawar juzu'i cikin sauri, kuma za'a iya kammala haɗin cDNA na farko a 42°C cikin mintuna 15.
Tsarin jujjuyawar wannan kit ɗin kuma zai iya kula da kyakkyawan aiki a 50°C.Don haka, ya fi fa'ida ga hadaddun samfuran RNA kuma yana iya sauƙin rubuta samfuran RNA tare da babban abun ciki na GC da hadadden tsarin sakandare.
Abubuwan Kit
| 5× gDNase Mix |
| 5× RO-SaukiTMMix |
| RNase-Free ddH2O |
| Umarni |
Fasaloli & fa'idodi
-Ingantacciyar ikon cire gDNA, wanda zai iya cire gDNA a cikin samfuri a cikin mintuna 2.
-Ingantaccen tsarin jujjuya rubutu, yana ɗaukar mintuna 15 kawai don kammala haɗin layin farko na cDNA.
-Complex samfuri: samfura tare da babban abun ciki na GC da hadaddun tsarin sakandare kuma ana iya juyawa tare da ingantaccen inganci.
-Tsarin jujjuya juzu'i mai girma, samfuran pg-matakin kuma na iya samun cDNA mai inganci.
-Tsarin rubutun baya yana da babban kwanciyar hankali na thermal, mafi kyawun zafin jiki shine 42℃, kuma har yanzu yana da kyakkyawan aikin juzu'i a 50℃.
Kit aikace-aikace
An yi amfani da shi kai tsaye a cikin PCR na Real Time don tantance maganganun kwayoyin halitta.
Yana iya sauri da kuma daidai tantance alamar RNA kamar kwayar cutar RNA.
Juya rubutun samfuran RNA tare da babban abun ciki na GC ko hadadden tsarin sakandare.
Gudun aiki
Adana da Rayuwar Shelf
Ya kamata a adana kayan a -20°C.Ajiye samfurin a cikin firiji mai yawan zafin jiki a -20°C nan da nan bayan an karɓa.Idan yanayin ajiya ya dace, samfur ɗin ba zai ƙasƙantar da kowane aiki ba yayin lokacin ingancin shekara 1.
