Samar da ODM China CE Amincewa da Nucleic Acid Rna DNA Haɓaka da Tsabtace Kayan Keɓancewa na Reagent don Gano PCR
Bayanin Kit:
Cikin sauri da inganci cire tsafta mai inganci da jimillar RNA daga kyallen dabbobi daban-daban.
Babu buƙatar damuwa game da lalata RNA.Duk tsarin ba shi da RNase-Free
Cire DNA yadda yakamata ta amfani da Rukunin Tsabtace DNA
Cire DNA ba tare da ƙara DNA ba
Sauƙaƙe-dukkan ayyukan ana kammala su a cikin zafin jiki
Ana iya kammala aiki mai sauri a cikin mintuna 30
Amintacciya — ba a yi amfani da reagent Organic ba
Babban tsarki-OD260/280≈1.8-2.1
Tare da tabbatacce da kuma ci gaba hali ga abokin ciniki ta sha'awa, mu kamfanin ci gaba da inganta mu samfurin ingancin saduwa da bukatun abokan ciniki da kuma kara mayar da hankali a kan aminci, AMINCI, muhalli bukatun, da kuma} ir} na Supply ODM China CE Amincewa da Nucleic Acid Rna DNA hakar da tsarkakewa Reagent keɓe Kits for PCR Gane, Muna tunanin za mu zama jagora a cikin high quality- da kuma kasa da kasa kayayyakin.Muna fatan yin aiki tare da ƙarin abokai don moriyar juna.
Tare da ingantacciyar hali da ci gaba ga sha'awar abokin ciniki, kamfaninmu yana ci gaba da haɓaka ingancin samfuranmu don saduwa da bukatun abokan ciniki kuma yana mai da hankali kan aminci, aminci, buƙatun muhalli, da haɓaka sabbin abubuwa.Sin Nucleic Acid keɓe Kit, Warewa Acid Nucleic Acid, Muna da suna mai kyau ga barga ingancin kayayyakin, da samu da abokan ciniki a gida da kuma kasashen waje.Kamfaninmu zai jagoranci ta hanyar ra'ayin "Tsaya a Kasuwannin Gida, Tafiya zuwa Kasuwan Duniya".Muna fata da gaske cewa za mu iya yin kasuwanci tare da masu kera motoci, masu siyar da motoci da galibin abokan aikinmu na gida da waje.Muna sa ran hadin kai na gaskiya da ci gaba tare!
Bayanin Kits
Shirye-shirye 50, Shirye-shiryen 200
Wannan kit ɗin yana amfani da ginshiƙi da dabarar da kamfaninmu ya samar, wanda zai iya fitar da tsabta mai tsabta da inganci mai kyau na RNA daga nau'in nau'in dabba daban-daban tare da babban inganci.Yana samar da ingantaccen DNA-Cleaning Column, wanda zai iya raba da kuma adsorb DNA na genomic daga supernatant da lysate nama, mai sauƙi da ceton lokaci;Rukunin RNA-kawai na iya ɗaure RNA da kyau da kyau kuma ana iya sarrafa su lokaci guda tare da keɓancewar dabara Yawancin samfurori.
Dukkanin tsarin ba shi da RNase-Free, don kada RNA da aka fitar ba ta lalacewa ba;Buffer RW1, Tsarin wanke buffer RW2, ta yadda RNA da aka samu ba ta da furotin, DNA, ion, da gurɓataccen mahalli.
Abubuwan kayan aiki:
| Buffer RL1, Buffer RL2 |
| Buffer RW1, Mai Buffer RW2 |
| RNase-Free ddH2O, Rukunin Tsabtace DNA |
| Rukunin RNA-Kawai |
Fasaloli & fa'idodi
■ Babu buƙatar damuwa game da lalacewar RNA;Duk tsarin ba shi da RNase-Free
∎ Cire DNA da kyau ta amfani da Rukunin Tsabtace DNA
■ Cire DNA ba tare da ƙara DNA ba
■ Ana kammala ayyuka masu sauƙi-duk a yanayin zafin ɗaki
■ Ana iya kammala aikin gaggawa cikin mintuna 30
∎ Amintacciya-babu reagent na halitta da ake buƙata
■ Babban tsarki -OD260/280≈1.8-2.1
Kit aikace-aikace
Ya dace da hakar da tsarkakewar jimillar RNA daga sabo ko daskararru iri-iri na kyallen dabbobi ko sel masu al'ada.
Siffofin samfur
∎ Aikace-aikace na ƙasa: haɗin cDNA na farko, RT-PCR, cloning na kwayoyin halitta, Northern Blot, da sauransu.
Samfurori: kyallen jikin dabba, ƙwayoyin al'ada
■ Adadin: Nama 10-20mg, Kwayoyin (2-5) × 106
■ Ƙarfin ɗaurin DNA na ginshiƙin tsarkakewa: 80 μg
■ Ƙarfin haske: 50-200 μl
Gudun aiki
zane
Jimlar Kayan Keɓewar Dabbobin RNA da aka yi maganin 20mg
Sabbin samfuran linzamin kwamfuta, ɗauki 5% tsarkakakken jimlar RNA 1% agar
Glycogel electrophoresis
1: Baki 2: Koda
3: Hanta 4: Zuciya
Adana da rayuwar shiryayye
Ana iya adana kit ɗin na tsawon watanni 24 a zazzabi na ɗaki (15-25 ℃) ko 2-8 ℃ na tsawon lokaci.Buffer RL1 za a iya adana a 4 ℃ ga 1 watan bayan ƙara β-mercaptoethanol (na zaɓi) .Tare da tabbatacce da kuma ci gaba hali ga abokin ciniki ta sha'awar, mu kamfanin ci gaba da inganta mu samfurin ingancin saduwa da bukatun abokan ciniki da kuma kara mayar da hankali a kan aminci, AMINCI, muhalli bukatun, da kuma sabon abu na Supply ODM China CE Amincewa da Nucleic Acid ANC Rnaction PC. za mu zama jagora wajen bunkasawa da samar da kayayyaki masu inganci da mafita a kasuwannin kasar Sin da na kasa da kasa.Muna fatan yin aiki tare da ƙarin abokai don moriyar juna.
Samar da ODMSin Nucleic Acid keɓe Kit, Warewa Acid Nucleic Acid, Muna da suna mai kyau ga barga ingancin kayayyakin, da samu da abokan ciniki a gida da kuma kasashen waje.Kamfaninmu zai jagoranci ta hanyar ra'ayin "Tsaya a Kasuwannin Gida, Tafiya zuwa Kasuwan Duniya".Muna fata da gaske cewa za mu iya yin kasuwanci tare da masu kera motoci, masu siyar da motoci da galibin abokan aikinmu na gida da waje.Muna sa ran hadin kai na gaskiya da ci gaba tare!
Ba a fitar da RNA ko amfanin RNA yayi ƙasa
Sau da yawa akwai dalilai iri-iri waɗanda ke shafar ingancin farfadowa, kamar: samfurin nama na RNA abun ciki, hanyar aiki, ƙarar haɓaka, da sauransu.
1. Ice wanka ko cryogenic (4 ° C) centrifugation aka yi a lokacin aiki.
Shawarwari: Yi aiki a dakin da zafin jiki (15-25 ° C) a duk tsawon tsari, kada ku yi wanka da centrifuge a ƙananan yanayin zafi.
2. Ƙimar samfurin da ba daidai ba ko yawan lokacin ajiyar samfurin.
Shawarwari: Ajiye samfurori a -80 ° C ko daskare a cikin ruwa nitrogen kuma kauce wa daskare-narke maimaita amfani;yi ƙoƙarin amfani da sabobin nama ko ƙwayoyin al'ada don hakar RNA.
3. Rashin isasshen samfurin lysis.
Shawarwari: Lokacin da aka haɗa nama, tabbatar da cewa nama ya isa daidai kuma cewa ƙwayoyin nama sun rabu sosai don bayyana sakin RNA.
4. Ba a ƙara eluent daidai ba.
Shawarwari: Tabbatar da cewa RNase-Free ddH2O ana ƙara juzu'i zuwa tsakiyar membrane ginshiƙin tsarkakewa.
5. Ba a ƙara madaidaicin ƙarar cikakken ethanol zuwa Buffer RL2 ko Buffer RW2 ba.
Shawarwari: Bi umarnin, ƙara madaidaicin ƙarar cikakkar ethanol zuwa Buffer RL2 da Buffer RW2 kuma gauraya da kyau kafin amfani da kit ɗin.
6. Nama samfurin sashi bai dace ba.
Shawarwari: Yi amfani da 10-20 MG na nama ko (1-5) × 106Kwayoyin a cikin 500 μl buffer RL1, saboda yawan amfani da nama zai iya haifar da raguwar hakar RNA.
7. Rashin ingantaccen ƙarar ƙyalli ko rashin cikawa.
Shawarwari: Girman haɓakar ginshiƙin tsarkakewa shine 50-200 μl;idan tasirin elution bai gamsar ba, ana ba da shawarar tsawaita lokacin sanyawa dakin zafin jiki bayan ƙara preheated RNase-Free ddH2O, misali na 5-10 min.
8.Shafin tsarkakewa yana da ragowar ethanol bayan wankewar buffer RW2.
Shawarwari: Idan akwai ragowar ethanol bayan wankewar Buffer RW2, centrifugation mara amfani na 1min, lokacin aikin centrifugation na bututu mara kyau za'a iya ƙara zuwa 2min, ko kuma ana iya sanya ginshiƙin tsarkakewa a dakin da zafin jiki na 5 min don cire isasshen ethanol.
RNA mai tsafta ya lalace
Ingancin RNA da aka tsarkake yana da alaƙa da abubuwa kamar adana samfurin, gurɓataccen RNase, da magudi, da sauransu.
1. Ba a adana samfuran nama a cikin lokaci.
Shawarwari: Idan ba a yi amfani da samfurori na nama ko sel a cikin lokaci ba bayan tattarawa, nan da nan a adana a -80 ° C ko nitrogen mai ruwa.Don cire RNA, yi amfani da sabon samfurin nama ko tantanin halitta a duk lokacin da zai yiwu.
2. Maimaita daskare-narke samfuran nama.
Shawarwari: Lokacin adana samfuran nama, yana da kyau a yanke su kanana don adanawa, kuma a cire ɗaya daga cikin guntun lokacin amfani da su don guje wa daskare-narke samfurin da kuma lalata RNA.
3. Ana gabatar da RNase ko a'a sanye da safar hannu, abin rufe fuska, da sauransu yayin aikin.
Shawarwari: Gwajin cirewar RNA an fi yin su a cikin ɗakunan sarrafa RNA daban kuma an share teburin kafin gwajin.
Sanya safar hannu da abin rufe fuska yayin gwajin don rage lalata RNA wanda ya haifar da gabatarwar RNase.
4. Reagents sun gurbata da RNase yayin amfani.
Shawarwari: Sauya da sabon Kit ɗin Keɓewar Dabbobi na RNA don gwaje-gwaje masu alaƙa.
5. Bututun centrifuge, tukwici, da sauransu da ake amfani da su wajen sarrafa RNA sun gurɓata da RNase.
Shawarwari: Tabbatar da cewa bututun centrifuge, tukwici, pipettes, da sauransu. da ake amfani da su wajen cire RNA duk ba su da RNase.
RNA da aka tsarkake yana shafar gwaje-gwaje na ƙasa
RNA da aka tsarkake ta hanyar ginshiƙin tsarkakewa, idan ions gishiri, abun cikin furotin ya yi girma zai shafi gwajin ƙasa, kamar: juzu'i na baya, Northern Blot et al.
1. RNA da aka elutioned yana da ragowar ion gishiri.
Shawarwari: Tabbatar da cewa an ƙara madaidaicin ƙarar ethanol zuwa Buffer RW2 kuma kuyi ginshiƙan tsarkakewa guda 2 a saurin centrifugal da aka nuna don aiki;idan akwai ragowar ion gishiri, bar ginshiƙin tsarkakewa zuwa Buffer RW2 na 5 min a zafin daki kuma yi centrifugation don haɓaka kawar da gurɓataccen gishiri.
2. Ethanol saura a cikin elutioned RNA.
Shawarwari: Tabbatar da cewa bayan buffer RW2 wankewa, yi aikin centrifugation mara amfani a cikin saurin centrifugation da aka nuna don aiki, ƙara lokacin aikin centrifugation mara amfani zuwa 2 min idan har yanzu akwai ragowar ethanol, ko barin shi a cikin dakin da zafin jiki na 5 min bayan fanko tube centrifugation don ƙara yawan cirewar ethanolue.
