Sarrafa-CoV-2 Kayan Gane Acid Nucleic (Tsarin Binciken Fluorescent PCR Multiplex)
Bayanin Kit:
PCR kai tsaye:Babu buƙatar kayan cirewar acid nucleic da Tsarin, an kammala samfurin 96 a cikin awa 1 ko makamancin haka.
◮ Ƙananan buƙatun kayan aiki da sauƙin amfani:Kawai buƙatar tsarin PCR na Real-Time .
◮ LoD da babban hankali:Hankalin ganowa zai iya kaiwa mafi ƙanƙanta matakin kamar kwafi 500/ml.
Amfani da Niyya
Ka'idar gwaji
An ƙirƙira wannan ƙirar farko da saitin bincike don gano jerin abubuwan da aka adana na SARS-CoV-2 (Genen ORF1ab, N gene da E gene).Kit ɗin ya ƙunshi Ikon Ciki wanda ake amfani dashi don tantance ingancin samfur.Fasahar PCR ta Real-Time tana amfani da nau'in sarkar polymerase (PCR) don haɓaka takamaiman jeri na manufa da takamaiman bincike don gano RNA haɓakawa.An yiwa masu binciken lakabi da mai ba da labari mai kyalli da rini mai kashewa.
Cikakken Bayani
◮Ƙayyadaddun bayanai:48 Rxns/kit, 96 Rxns/kit
◮ Takamaiman:96.72%
Hankali:97.92%
Abubuwan da aka gyara
| A'a. | Bangaren | Adadin | Babban sashi | |||
| 48 rxn | 96 rxn | |||||
| 1 | Nucleic acid sakiwakili | 1.5 ml / tube | 1 tube | 1.5 ml / tube | 1 tube | Surfactant |
| 2 | Mai kare RNA | 27 μl / tube | 1 tube | 53 μl / tube | 1 tube | Mai hana RNase |
| 3 | SARS-CoV-2dauki bayani | 800 μL / tube | 1 tube | 1600 μL / tube | 1 tube | Firamare, bincike, mayar da martani, dNTP |
| 4 | SARS-CoV-2cakuda enzyme | 80 μl / tube | 1 tube | 160 μl / tube | 1 tube | Hot fara Taq enzyme,M-MLV enzyme |
| 5 | SARS-CoV-2tabbatacce iko | 100 μL / tube | 1 tube | 100 μL / tube | 1 tube | Recombinant plasmid dauke da manufararrabuwa, RNA |
| 6 | SARS-CoV-2korau iko | 1200 μL / tube | 1 tube | 1200 μL / tube | 1 tube | TE buffer |
Fasaloli & fa'idodi
Yadda ake amfani da shi
Hanyar 1: Hanyar PCR kai tsaye
Hanyar 2: Kwayar cuta ta RNA Keɓewa + PCR
Hanyar 1: Hanyar PCR kai tsaye ta amfani daSamfurin Sakin Wakilin
Hanyar ganowa
Jirgin Jirgin Ruwa da Ajiye
An rufe shi daga haske kuma an adana shi a -20 ± 5 ℃;
Rayuwar rayuwa
shekara 1
Bayanin oda
