SARS-CoV-2 Bambance-bambancen Nucleic Acid Gane Kit II (Tsarin Binciken Fluorescent PCR Multiplex) - don gano bambance-bambancen daga Burtaniya, Afirka ta Kudu, Brazil, da Indiya
Bayanin Kit:
PCR kai tsaye:Babu buƙatar kayan cirewar acid nucleic da Tsarin, an kammala samfurin 96 a cikin awa 1 ko makamancin haka.
◮ Ƙananan buƙatun kayan aiki da sauƙin amfani:Kawai buƙatar tsarin PCR na Real-Time .
◮ LoD da babban hankali:Hankalin ganowa zai iya kaiwa mafi ƙanƙanta matakin kamar kwafi 500/ml.
Ƙayyadaddun bayanai
48 Rxns/kit, 96 Rxns/kit
| A'a. | Bangaren | Adadin | Babban sashi | |||
| 48 rxn | 96 rxn | |||||
| 1 | Nucleic acid wakili saki | 1.4 ml / tube | 2 bututu | 5.3 ml / kwalba | kwalba 1 | Surfactant |
| 2 | Mai kare RNA | 53 μl / tube | 1 tube | 106 μL / tube | 1 tube | Mai hana RNase |
| 3 | SARS-CoV-2 Bambanci Maganin amsawa A | 800 μL / tube | 1 tube | 1600 μL / tube | 1 tube | Firamare, bincike, mayar da martani, dNTP |
| 4 | SARS-CoV-2 Bambanci Maganin amsawa B | 800 μL / tube | 1 tube | 1600 μL / tube | 1 tube | Firamare, bincike, mayar da martani, dNTP |
| 5 | SARS-CoV-2 enzyme cakuda | 160 μl / tube | 1 tube | 320 μL / tube | 1 tube | Hot fara Taq enzyme, M-MLV enzyme |
| 6 | SARS-CoV-2 tabbatacce iko | 100 μL / tube | 1 tube | 100 μL / tube | 1 tube | Recombinant plasmid dauke da guntun manufa, RNA |
| 7 | SARS-CoV-2 korau iko | 1200 μL / tube | 1 tube | 1200 μL / tube | 1 tube | TE buffer |
Lura: Ba za a iya amfani da sassa daban-daban daga kayan aiki daban-daban ba.
Fasaloli & fa'idodi:
Mai sauri da sauƙi: kammala gwajin cikin kusan awa 1
Guji asarar nucleic acid: babu buƙatar tsarkakewar acid nucleic
Ƙarfin ganowa da babban hankali, musamman suitabe don babban saurin ganowa
Ƙananan buƙatun kayan aiki da sauƙin amfani sosai
Ganewar hankali na iya kaiwa mafi ƙanƙanta matakin kamar kwafi 500/ml).
Yadda ake amfani da:
1.Mai amfani da kayan aiki
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
2.Tsarin ganowa
Samfurin sarrafawa: Naopharyngeal ko swab samfurin swab (aiki 1 kawai)Jira minti 10)
PCR amplificaton<= 55min)
Gudun Aiki:
Ajiya:
An rufe shi daga haske kuma an adana shi a -20 ± 5 ℃;
Ajiye a 2-8 ℃ bayan buɗewa, amfani a cikin kwanaki 7;guji maimaita daskarewa (< sau 5)
Rayuwar rayuwa:shekara 1
