QuickEasyᵀᴹ Real Time PCR Kit-Taqman
Bayanin Kit:
◮Tsarin ingantawa na musamman na PCR yana sa 2 × QuickEasyTMReal PCR Mix-Taqman mafi dacewa.
◮Hot-star Foregene HS Taq Polymerase yana da ingantaccen haɓakawa, haɓaka haɓakawa, da ƙayyadaddun haɓakawa mafi girma.
◮2× Mai SauƙiTMReal PCR Mix -Taqman yana amfani da tsari na musamman wanda Foregene ya inganta don haɓaka hankali da ƙayyadaddun gano takamaiman bincike na jeri, wanda za'a iya amfani da shi don ƙididdigewa da kwafin bambance-bambancen lamba da samun ingantaccen sakamako.
◮Wannan samfurin ya zo tare da rini na tunani na ciki na ROX, wanda za'a iya amfani dashi don kawar da bayanan sigina da kuskuren sigina tsakanin rijiyoyi, wanda ya dace da abokan ciniki don amfani da nau'ikan kayan aikin PCR masu yawa.
Bayani
2 × QuickEasyTMReal PCR Mix-Taqman wanda QuickEasy ke bayarwaTMKit-Taqman na Real Time PCR Kit-Taqman sabon tsarin haɗin gwargwado ne wanda aka tsara don haɓaka haɓaka PCR na Real Time ta amfani da takamaiman bincike na kyalli.Babban sa na Foregene HS Taq DNA Polymerase ya dogara ne akan gyaran Antibody, wanda aka yi amfani dashi tare da Foregene yana haɓaka ingantaccen tsarin PCR, yana da ƙayyadaddun ƙayyadaddun ƙayyadaddun haɓakawa da haɓakawa.Idan aka kwatanta da mahaɗin PCR na yau da kullun, yana da ƙarfin ƙara ƙarfi da ƙarancin daidaituwa.Ana iya amfani da shi a cikin amsawar PCR na fluorescence mai ƙididdigewa don rage ƙayyadaddun haɓakawa da haɓaka daidaiton PCR.Zai iya inganta ƙayyadaddun samfur da halayen amsawa.A lokaci guda, an ba da ROX azaman rini na tunani na ciki.
The QuickEasyTMKit-Taqman na Real Time PCR Kit-Taqman yana da fa'idar aikace-aikace zuwa adadin samfuri, kuma yana iya samun ingantacciyar ma'auni mai kyau a yankuna daban-daban masu ƙididdigewa, kuma yana iya ƙididdigewa da gano ƙwayoyin halittar da aka yi niyya tare da maimaituwa mai ƙarfi da ƙarfin gwiwa.
Ƙayyadaddun bayanai
200 Preps,500 Preps,1000 Preps,2000 Preps
Abubuwan Kit
| SauƙaƙeTMReal Time PCR Kit-Taqman(20μltsarin) | ||||
| Abubuwan da ke ciki | QP-01121 | QP-01122 | QP-01123 | QP-01124 |
| 200 Preps | 500 Preps | 1000 Preps | 2000 Preps | |
| 2×Mai sauriSauƙiTM Farashin PCR Mix-Taqman | 1 ml×2 | 1.7 ml×3 | 1.7 ml×6 | 1.7 ml×12 |
| 20×ROX Reference Dye | 200μL | 0.5 ml | 1 ml | 1 ml×2 |
| DNase-Free ddH2O | 1.7 ml | 1.7 ml×2 | ml 10 | 20 ml |
| IFU | 1yanki | 1yanki | 1yanki | 1yanki |
Fasaloli & fa'idodi
∎ Tsarin inganta PCR na musamman yana sa 2×QuickEasyTMReal PCR Mix-Taqman mafi dacewa.
■ Hot-star Foregene HS Taq Polymerase yana da ingantaccen haɓakawa, haɓakar haɓakawa, da ƙayyadaddun ƙayyadaddun haɓakawa.
■ 2× SauƙaƙeTM Real PCR Mix -Taqman yana amfani da tsari na musamman wanda Foregene ya inganta don haɓaka hankali da ƙayyadaddun gano takamaiman bincike na jeri, wanda za'a iya amfani da shi don ƙididdigewa da kwafin bambance-bambancen lamba da samun ingantaccen sakamako.
n Wannan samfurin ya zo tare da rini na ciki na ROX, wanda za'a iya amfani dashi don kawar da bayanan sigina da kuskuren sigina tsakanin rijiyoyi, wanda ya dace da abokan ciniki don amfani da nau'ikan kayan aikin PCR daban-daban.
Kit aikace-aikace
n Fluorescence ƙididdige PCR don samfurin ƙididdiga na ƙididdigewa;
■ Cna yau da kullun PCR;
■ Ana iya amfani da su don gano allele.
Adana da Rayuwar Shelf
Ya kamata a adana kit ɗin a -20 ° C a cikin duhu;idan ana amfani dashi akai-akai, ana iya adana shi a zazzabi na 4 ° C na ɗan gajeren lokaci (amfani da shi cikin kwanaki 10).
Ka'idodin ƙira na ainihin lokacin PCR
Gaba Farawa da Juya Farko
Don Real Time PCR, ƙirar farko tana da mahimmanci.Abubuwan haɓaka suna da alaƙa da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙimar PCR, kuma ana iya tsara su tare da la'akari da ƙa'idodi masu zuwa:
Tsayin farko: 18-30bp.
GC abun ciki: 40-60%.
Ƙimar Tm: Software na ƙira na farko, kamar Primer 5, na iya ba da ƙimar Tm na firamare.Ma'auni na Tm na sama da na ƙasa ya kamata su kasance kusa da iyawa.Hakanan ana iya amfani da dabarar lissafin Tm: Tm = 4 °C (G + C) + 2 °C (A + T).Lokacin yin PCR, ana zaɓar zafin da ke ƙasa da ƙimar ƙimar Tm na 5 ° C gabaɗaya azaman zazzabi mai ɗaukar nauyi (madaidaicin haɓakar zafin jiki na iya ƙara ƙayyadaddun halayen PCR).
Alamar farko da samfuran PCR:
Tsawon samfurin PCR na ƙirar ƙira ya fi dacewa 100-150bp.
Ya kamata a kauce wa ƙera ƙirar ƙira a cikin yanki na biyu na samfuri gwargwadon yiwuwa.
Guji samuwar tushe guda 2 ko sama da haka tsakanin 3′ na ƙofofin sama da na ƙasa.
Babban tushe 3′ na tashar ba zai iya kasancewa tare da ƙarin ƙarin 3 a jere G ko C.
Masu farawa da kansu ba za su iya samun ƙarin tsari ba, in ba haka ba za a kafa tsarin gashin gashi, yana shafar haɓaka PCR.
Yakamata a rarraba ATCG daidai gwargwado kamar yadda zai yiwu a cikin jeri na farko, kuma ya kamata a guje wa tushe na 3′ a matsayin T.
Shafi 1: Tantanin halitta Direct RT-qPCR Kit na ƙarin fakitin
1.Cell Lysis Magani
|
| |||
| Abubuwan Kit (24-riji tsarin lysis / rijiyar) | Saukewa: DRT-01011-A1 | Saukewa: DRT-01011-A2 | |
| 100 T | 500 T | ||
| SasheI | Buffer CL | 20 ml | 100 ml |
| Foregene Protease Plus II | 400 ml | 1 ml × 2 | |
| Buffer ST | 1 ml × 2 | 10 ml | |
| SasheII | Goge DNA | 400 ml | 1 ml × 2 |
2.RT Mix
|
| |
| Abubuwan Kit (20 μl tsarin amsawa) | Saukewa: DRT-01011-B1 |
| 200 T | |
| 5× Direct RT Mix | 800 ml |
| RNase-Free ddH2O | 1.7 ml × 2 |
|
| ||
| Abubuwan Kit (20 μl tsarin amsawa) | Saukewa: DRT-01011-C1 | Saukewa: DRT-01011-C2 |
| 200 T | 1000 T | |
| 2× Direct qPCR Mix-SYBR | 1 ml × 2 | 1.7 ml × 6 |
| 50× ROX Reference Dye | 40ml ku | 200 μl |
| RNase-Free ddH2O | 1.7 ml | 10 ml |
-
Buccal Swab/FTA Katin DNA keɓe Kit Genomic ...
-
Shuka iri (Polysaccharide Polyphenol mai arziki, smal ...
-
Tsarin Gano DNA na Foregene 38Y-C (Case)
-
Kifi na Zebra Direct Kit-UNG PCR Lysis kai tsaye ...
-
Kit ɗin keɓantawar DNA na Kwanciyar Hannun Cirar DNA Tsarkakewa...
-
MALT1 Dual Color Break Apart bincike
