Labaran Masana'antu
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Ƙayyadaddun ƙira na farko-ganewa, kwanciyar hankali da haɓaka haɓakawa
Ƙayyadaddun ganowa A mafi yawan lokuta, manufar ƙira ta farko ita ce haɓaka takamaiman PCR.Ana ƙayyade wannan ta ƙarin ko žasa tasirin da ake iya faɗi na yawancin masu canji.Ɗaya mai mahimmanci mai mahimmanci shine jerin a ƙarshen 3' na farko.Mahimmanci, ƙididdigar PCR da aka tsara don ...Kara karantawa -
Magungunan Nucleic acid sun shiga zamanin zinare, menene mahimman fasahohin da ke buƙatar warwarewa cikin gaggawa?
Source: Medical Micro Bayan fashewar COVID-19, an amince da rigakafin mRNA guda biyu don tallata da sauri, wanda ya jawo hankali sosai ga haɓaka magungunan nucleic acid.A cikin 'yan shekarun nan, adadin magungunan nucleic acid da ke da yuwuwar zama magungunan blockbuster sun buga ...Kara karantawa -
Nazarin Tabbataccen Fihirisar Bincike na Farko a Lokacin Farko na Reagents na PCR
Tabbatar da aikin firamare da bincike a farkon matakin PCR reagents da kuma ƙayyade mafi dacewa halayen halayen sune abubuwan da ake buƙata don tabbatar da ingantaccen ci gaba na gwaje-gwaje na yau da kullun.Don haka ta yaya muke buƙatar tabbatar da binciken farko a farkon…Kara karantawa -
Samfuran abubuwan ilimin da masu dubawa dole ne su gani
Gwajin dakin gwaje-gwaje yana farawa da tarin samfuri, kuma tarin samfurin shine mafi sauƙi don kau da kai.Abu mafi mahimmanci don tarin samfurin shine zaɓar nau'in samfurin daidai, amfani da kayan aikin samfurin da suka dace, da gudanar da sufuri da sarrafawa.I.Sample type Common sam...Kara karantawa -
Magani na ƙarshe ga gurɓataccen aerosol na nucleic acid
Hanyoyin PCR da gurɓataccen iska a cikin dakunan gwaje-gwaje na acid nucleic kamar ɓangarori biyu ne na tsabar kuɗi.Za mu iya zaɓar samun shi ne kawai ko a'a, amma ba za mu iya zaɓar ko muna so ko mu kashe shi ba.1. Nuna mai cire DNA Don cimma nasarar cire sararin samaniya...Kara karantawa -
Saurin gano tsire-tsire na transgenic
A matsayin sabon abu a cikin dakin gwaje-gwaje, ba aiki mai kyau ba ne don tantance shuke-shuke masu kyau daga gungun shuke-shuke tare da ƙarancin juyawa.Da fari dai, dole ne a fitar da DNA daga ɗimbin samfuri ɗaya bayan ɗaya, sannan PCR za ta gano kwayoyin halittar waje.Koyaya, sakamakon yawanci ba komai bane...Kara karantawa -
Cikakken fassarar mahimman abubuwan gwajin nucleic acid na SARS-CoV-2, me yasa ake samun rashin ƙarfi na ƙarya da sake gwadawa?
A farkon matakin barkewar cutar, saboda saurin ci gaba, saurin gano marasa lafiya da ake zargi shine mabuɗin hana COVID-19.Wasu da aka amince da sake gano abubuwan gano acid nucleic suna da ɗan gajeren lokacin haɓakawa, kuma akwai matsaloli kamar tabbatar da gaggawar aiki, rashin isasshen rea ...Kara karantawa -
Menene SNP?Maudu'ai akan Genetics na Yawan Jama'a
Haruffa uku SNP suna da yawa a cikin nazarin kwayoyin halittar jama'a.Ba tare da la'akari da binciken cutar ɗan adam ba, matsayi na dabi'ar amfanin gona, juyin halittar dabba da ilimin halittu, ana buƙatar SNPs azaman tushe.Duk da haka, idan ba ku da zurfin fahimtar kwayoyin halitta na zamani ...Kara karantawa -
Yadda ake haɓaka ingancin juzu'i na LncRNA?
Siffofin lncRNA: 1. lncRNAs yawanci sun fi tsayi, tare da magana mai ƙarfi da hanyoyin rarraba daban-daban yayin rarrabuwa;2. Idan aka kwatanta da codeing genes, lncRNA yawanci ƙasa;3. Yawancin lncRNAs suna da ƙayyadaddun bayanan lokaci da sarari a cikin tsari ...Kara karantawa -
Babban Magani Hudu Don Kula da Gurɓatar Samfuran PCR
1: Sauya kayan gwaji a cikin lokaci Saita (NTC) iko mara kyau kuma maimaita shi sau da yawa.Da zarar an gano cewa akwai gurɓataccen samfurin PCR a cikin dakin gwaje-gwaje, maye gurbin duk kayan gwaji a cikin lokaci.Kamar: sake tsarkewa da shirya abubuwan farko, sake bakara tip ɗin pipette, E ...Kara karantawa -
Biyu-dual-aiki na RT-PCR enzymes
Rubutun juzu'i na al'ada ba zai iya jure yanayin zafi mai girma ba (mafi kyawun zafin jiki don ayyukan MMLV shine 37-50°C, kuma AMV shine 42-60°C).RNA mafi hadaddun ƙwayar cuta ba za a iya jujjuya shi yadda ya kamata zuwa cDNA a ƙananan zafin jiki ba, yana haifar da ingantaccen ganowa Ragewar.Ta...Kara karantawa -
Nucleic acid isothermal haɓaka fasahar haɓakawa
PCR ita ce fasahar haɓaka haɓakar acid nucleic da aka fi amfani da ita kuma ana amfani da ita sosai saboda azanci da ƙayyadaddun sa.Koyaya, PCR yana buƙatar maimaita ƙarancin zafi kuma ba zai iya kawar da iyakokin dogaro da kayan aiki da kayan aiki ba, wanda ke iyakance aikace-aikacen sa a cikin asibiti ...Kara karantawa
