lncRNA fasali:

1. lncRNAs yawanci sun fi tsayi, tare da magana mai ƙarfi da hanyoyin rarraba daban-daban yayin rarrabuwa;

2. Idan aka kwatanta da codeing genes, lncRNA yawanci ƙasa;

3. Yawancin lncRNAs suna da ƙayyadaddun magana na ɗan lokaci da sarari a cikin tsarin bambance-bambancen nama da haɓakawa;

4. Akwai maganganun halaye a cikin ciwace-ciwacen daji da sauran cututtuka;

5. Wuraren ƙananan ƙwayoyin cuta na lncRNA sun bambanta;

6. Jerin yana da ƙasa a cikin kiyayewa, amma aikin yana da wani matakin kiyayewa da sauransu.

Maganin juyar da rubutun LncRNA:

Mun san cewa lncRNA yana da babban tsari a cikin sel.Irin wannan RNA sau da yawa yana da hadaddun sifofi irin su madaukai masu tushe ko ginshiƙan gashi.Idan tsarin na biyu na lncRNA ba za a iya warware shi yadda ya kamata ba, jujjuya juzu'i na iya yin rashin nasara ko rashin nasara yayin yin RT-qPCR.Matsalar ƙarancin inganci.

Gabaɗaya, akwai mahimman abubuwa guda uku a gare mu don samun samfuran cDNA masu inganci:

Ingancin samfurin RNA, juzu'i na juzu'i da jujjuya rubutu, da juyar da rubutun shine ainihin maɓalli.RNA da aka tsarkake ta hanyar reagents na hakar RNA da aka saba amfani da su sau da yawa yana ƙunshe da ragowar barasa da gishirin guanidine, waɗanda ke da tasirin hanawa mai ƙarfi akan mafi yawan bayanan baya;idan madaidaicin zafin jiki don juyar da martanin transcriptase yayi ƙasa, ba zai zama mai amfani ga RNA ba.Bude tsarin tsarin.

A halin yanzu, yanayin da ya dace don jujjuya bayanan da aka yi amfani da shi a yawancin dakunan gwaje-gwaje gabaɗaya 42 ° C ne, kuma matsakaicin zafin jiki na iya kaiwa kusan 50 ° C.A gaskiya ma, buɗe tsarin RNA mafi girma yana buƙatar zafin jiki mafi girma.

Yanayin zafin jiki don haɗa cDNA na farko na Fortune Biotech 2 × RT EasyTM Mix ya kai 50 ℃-65 ℃, wanda ke ba da damar buɗe babban matakin tsarin lncRNA gaba ɗaya, kuma lokacin aiki gajere ne.

Bugu da ƙari, samfurin kuma yana da ƙarfin hana tsangwama ga RNA tare da ragowar barasa da guanidine gishiri, wanda zai iya cika bukatun dakunan gwaje-gwaje don cimma babban juzu'i mai inganci.

Kit ɗin Rubutun cDNA na Farko

Abu na farko: RT-01011 (sau 25)

Siffofin:

1. Thermal kwanciyar hankali: 65 ℃ har yanzu yana da kyau reverse transcription aiki, wanda taimaka wajen bude hadaddun sakandare tsarin.

2. Haƙuri: har yanzu ana iya yin nasarar yin haɗin gwiwar cDNA akan samfuran RNA tare da ƙarancin tsabta (sauran gishiri na ethanol da guanidine)

3. Fast: aiki mai sauƙi, za a iya kammala haɗin cDNA na farko a cikin minti 25-35.

Aikace-aikace na ƙasa: PCR, qPCR, RACE, alamar guntu, ginin ɗakin karatu, da sauransu.

Bugu da ƙari, FJ Biotech yana ba da cikakken saiti na RT-PCR mafita don saduwa da bukatun nau'ikan abokan ciniki, gami da abubuwa masu zuwa:

Kit ɗin Haɗin CDNA Na Farko (Na qPCR)

Abu na farko: RT-01021 (sau 50)

Siffofin:

1. Sauƙaƙawa: An ƙara maƙallan rubutun juyi don sauƙaƙe aikin

2. Thermal kwanciyar hankali: 65 ℃ har yanzu yana da kyau reverse transcription aiki, wanda taimaka wajen bude hadaddun sakandare tsarin.

3. Haƙuri: Har yanzu ana iya yin nasarar yin haɗin gwiwar cDNA akan samfuran RNA tare da ƙarancin tsabta

4. Sensitivity: dace da ƙananan ƙididdiga samfurori na RNA

Aikace-aikacen ƙasa: An bayar da shi musamman don qPCR na gaba

Kit ɗin RT-PCR-mataki ɗaya

Abu na farko: RT-02011 (sau 100)

Siffofin:

1. Thermal kwanciyar hankali: 65 ℃ har yanzu yana da kyau reverse transcription aiki, wanda taimaka wajen bude hadaddun sakandare tsarin.

2. Haƙuri: Har yanzu ana iya yin nasarar yin haɗin gwiwar cDNA akan samfuran RNA tare da ƙarancin tsabta

3. Specificity: PCR MIX sanye take da kyau inganta haɓaka haɓakawa da ƙayyadaddun amsawa

Aikace-aikace na ƙasa: cDNA cloning, gwajin kwayoyin halitta da sauran gwaje-gwajen kwayoyin

Kit ɗin RT-PCR mai mataki biyu

Abu na farko: RT-02021 (sau 100)

Siffofin:

1. Sauƙi da sauri: amsawar bututu guda ɗaya

2. Thermal kwanciyar hankali: 65 ℃ har yanzu yana da kyau reverse transcription aiki, wanda taimaka wajen bude hadaddun sakandare tsarin.

3. Specificity: Foregene Taq da aka ƙara da kyau yana inganta ingantaccen haɓakawa da ƙayyadaddun halayen.

Aikace-aikace na ƙasa: gwaje-gwajen kwayoyin halitta kamar gwajin kwayoyin halitta

Kit ɗin RT-qPCR-mataki ɗaya-SYBR Green I

Abu na biyu: RT-02111 (sau 100)

Siffofin:

1. Sauƙaƙe: amsawar bututu guda ɗaya

2. Babban hankali: na iya gano ƙananan samfuran kwafi

3. Daidaituwa: dace da yawancin kayan kida masu kyalli

Aikace-aikace na ƙasa: gano samfuran RNA masu alama kamar ƙwayoyin cuta da ƙwayoyin cuta

Kit-Taqman mataki daya-mataki RT-qPCR

Abu na farko: RT-02131 (sau 100)

Siffofin:

1. Mai sauri: gano kwayar halittar da aka yi niyya

2. Babban hankali: na iya gano ƙananan samfuran kwafi

3. Daidaituwa: dace da yawancin kayan kida masu kyalli

Aikace-aikace na ƙasa: gano samfuran RNA masu alama kamar ƙwayoyin cuta da ƙwayoyin cuta
Kit ɗin Rubutun Forge Bioreverse na iya taimaka muku da kyau warware matsalar jujjuyawa.A lokaci guda, yana aiki tare da Forge Bio RNA Extraction Kit (wanda zai iya cire ragowar DNA yadda ya kamata) don samar muku da cikakkiyar maganin gwajin RNA.