RT-PCR fasaha ce da ta haɗu da juyar da rubutun RT na RNA da haɓaka sarkar polymerase (PCR) na cDNA.Fasahar RT-PCR tana da mahimmanci kuma mai amfani da ita, kuma ana iya amfani da ita don gano matakin maganganun kwayoyin halitta a cikin sel/nama, abun ciki na ƙwayoyin cuta na RNA a cikin sel, da cloning kai tsaye na jerin cDNA na takamaiman kwayoyin halitta.
AMFANIN KWANA:
Kit ɗin RT na Foregene yana da kwanciyar hankali mai ƙarfi a zazzabi na 50-65 ℃ (65°C na iya buɗe matakin na biyu na RNA don sanya martanin juzu'i ya ci gaba da kyau)
✱ Haƙuri mai ƙarfi, yana iya jurewa wasu ethanol da gishirin guanidine
| Sub-Jerin | Sunan samfur | Ƙayyadaddun bayanai | Lambar Catalog | Yanayin Ajiya |
| PCR Mix Series | 5ml ku | Saukewa: PH-01013 | -20 ℃ | |
| ml 40 | Saukewa: PH-01015 | |||
| 5ml ku | Saukewa: PH-01013-B | -20 ℃ | ||
| ml 40 | Saukewa: PH-01015-B | |||
| 1 ml | DP-20031 | -20 ℃ | ||
| 3 × 1.7 ml | DP-20032 | |||
| 3 × 1.7 ml | DP-20032-B | -20 ℃ | ||
| ml 50 | DP-20034-B | |||
| 2× Mai sauri PCR dauki premix tsarin 2× PCR Mix | 1 ml | DP-20041 | -20 ℃ | |
| 3 × 1.7 ml | DP-20042 | |||
| 500 × 20μl rxns | QP-01012 | -20 ℃ | ||
| 2000 × 20μl rxns | QP-01014 | |||
| 500 × 20μl rxns | QP-01022 | -20 ℃ | ||
| 2000 × 20μl rxns | QP-01024 | |||
| Jerin RT | 25× 20μl Ra'ayoyin | RT-01011 | -20 ℃ | |
| 100 × 20μl Amsa | RT-01012 | |||
| 200 × 10μl Amsa | RT-01022 | -20 ℃ | ||
| 800 × 10μl Amsa | RT-01023 | |||
| RT SauƙiTM II (Tare da gDNase) Jagora Premix don haɗakar cDNA na farko don PCR na ainihi tare da gDNase | 100 × 20μl Amsa | RT-01032 | -20 ℃ | |
| Lnc-RT JarumiTM I (Tare da gDNase) (Super Premix don haɗin cDNA na farko daga lncRNA) | 25 × 20μl Amsa | Saukewa: RTL-09098 | -20 ℃ | |
| 50 × 20μl Amsa | Saukewa: RTL-09099 | |||
| RT-PCR jerin | 100 rxn | RT-02011 | -20 ℃ | |
| 500 rxn | RT-02012 | |||
| 100 rxn | RT-02021 | -20 ℃ | ||
| 500 rxn | RT-02022 | |||
| RT-qPCR jerin | 100 rxn | RT-02111 | -20 ℃ | |
| 500 rxn | RT-02112 | |||
| 100 rxn | RT-02131 | -20 ℃ | ||
| 500 rxn | RT-02132 | |||
| Alamar DNA | 50 Shirye-shirye | Farashin DL-20011 | 4 ℃ | |
| 200 Preps | DL-20012 | |||
| 50 Shirye-shirye | DL-30011 | 4 ℃ | ||
| 200 Preps | DL-30012 |
