Ƙananan rabo na A260/A230 yawanci ana lalacewa ta hanyar ƙazanta tare da matsakaicin tsayin raƙuman ruwa a 230nm.Bari mu ga abin da waɗannan ƙazanta suka haɗa:

• Na kowa gurbacewa	Tsawon igiyar ruwa	Tasirin rabo
  Protein	~ 230nm da 280nm	Rage raguwar A260/A 280kuma A260/A 280rabo
  Guanidine gishiri	220-240 nm	Rage A260/A 280rabo
  Phenol	~ 270nm	-
  Trizol	~ 230nm da 270nm	Rage A260/A 280rabo
  EDTA	~ 230nm	Rage A260/A 280rabo
  Ethanol	230-240 nm	Rage A260/A 280rabo

 
 
 
Tsayin tsayin daka da ƙimar ƙimar gurɓataccen abu na gama gari

Pgurɓataccen rotein
Ana iya ɗaukar gurɓacewar furotin a matsayin gurɓataccen gurɓataccen abu a cikin tsarin hakar acid nucleic.Protein yana wanzu tsakanin babban ruwa mai ruwa da ƙasakwayoyin halittalokaci .Gurbacewa zai rage rabon A260/A280 da A260/A230 a lokaci guda, kuma rabon A260/A230 zai canza a fili fiye da rabon A260/A280.
A lokacin na gabajuyar da rubutuor qPCR halayen, ragowar furotin na iya hana ko tsoma baki tare da aikin enzyme.Hanya mafi kyau don guje wa gurɓataccen furotin ita ce a tuna da ka'idar "maimakon ƙasa da yawa, ƙananan adadin sau da yawa" lokacin da ake neman mai girma.

2. Guanidinium gurbatawa
hydrochloride (GuHCl) da guanidine thiocyanate (GTC) suna da tasiri na denaturing sunadaran, wanda zai iya sauri halakar da cell membranes a lokacin da nucleic acid hakar tsari, haifar da denaturation na gina jiki da hazo.Matsakaicin tsayin guHCl da GTC yana tsakanin 220-240 nm, kumaragowar guanidinium gishiri zai rage rabon A260/A230.Ko da yake ragowar guanidinium gishiri zai rage rabo,tasiri akan gwaje-gwajen da ke ƙasa a zahiri ba shi da komai.

3. Trizol gurbatawa
Babban bangaren Trizol shine phenol.Babban aikin phenol shine lyse Kwayoyin da saki sunadaran da nucleic acid abubuwa a cikin sel.Ko da yake phenol na iya sarrafa sunadaran haƙora da kyau, ba zai iya hana ayyukan RNase gaba ɗaya ba.Saboda haka, 8 -hydroxyquinoline, guanidine isothiocyanate, β-mercaptoethanol, da dai sauransu an kara zuwa TRIzol don hana endogenous da exogenous RNase.
Lokacin fitar da RNA ta salula, Trizol na iya saurin lyse sel kuma ya hana ƙwayar da aka saki daga sel, kuma ragowar Trizol zai rage yawan rabon A260/A230.
Hanyar sarrafawa: Lokacin centrifuging, dole ne a lura cewa phenol a cikin Trizol yana da sauƙin narkewa a cikin yanayin ruwa a ƙarƙashin yanayin 4 ° da zafin jiki.

4. Ethanol saura
Ana amfani da Ethanol a cikin tsari na ƙarshe don haɓaka DNA yayin da yake narkar da ions gishiri waɗanda za a iya ɗaure su da DNA.Tsayin tsayin daka na mafi girmasha kololuwarethanol kuma yana a 230-240 nm, wandaHakanan zai rage rabon A260/A230.
Hanyar guje wa ragowar ethanol za a iya maimaita sau biyu a lokacin elution na ƙarshe, busa a cikinhuma hudana mintuna biyu don ƙyale ethanol ya cika ƙafewa kafin ƙara maƙasudin don haɓakawa.
Koyaya, yakamata a san cewa rabon ƙima ne kawai na ƙimar ingancin RNA.Idan ayyukan da aka ambata a sama suna da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ma'auni, ba za su yi tasiri sosai kan gwaje-gwajen da ke ƙasa ba.
Samfura masu dangantaka:
Kit ɗin keɓewar dabbobi na jimlar RNA
Plant Total RNA keɓe kayan
Total Cell RNA keɓe kayan
Plant Total RNA keɓancewar kit Plus