Babban Ingancin China Babban Mai Haɓaka Manufacturer Bioer Bsc53 Magabio Plus Jimlar Kayan Tsabtace Rna
Bayanin Kit:
RNase-Free
Ana aiki da zafin jiki (15-25 ℃)
Tare da Rukunin Tsabtace DNA
Babban yawan amfanin RNA
Mai sauri:Gama hakar a cikin mintuna 11
Tsaro: Babu wani sinadari na halitta
Kyakkyawan inganci yana zuwa farawa da;sabis shine kan gaba;kungiyar ne hadin gwiwa" ne mu sha'anin falsafar da aka akai-akai lura da kuma bi da mu m for High Quality China High Efficiency Manufacturer Bioer Bsc53 Magabio Plus Total Rna tsarkakewa Kit, Mun yi imani da cewa mu dumi da kuma qualified taimako zai kawo muku m surprises da kuma arziki.
Kyakkyawan inganci yana zuwa farawa da;sabis shine kan gaba;ƙungiya ita ce haɗin gwiwa" shine falsafar kasuwancin mu wanda kamfaninmu ke kula da shi akai-akai kuma yana bi da shiChina Extraction Reagent, Magnetic Beads, Ta hanyar haɗawa da masana'antu tare da sassan kasuwancin waje, za mu iya ba da jimlar abokin ciniki mafita ta hanyar tabbatar da isar da samfuran da suka dace da kuma mafita zuwa wurin da ya dace a daidai lokacin, wanda ke goyan bayan abubuwan da muke da su da yawa, ƙarfin samarwa mai ƙarfi, daidaiton inganci, kayayyaki iri-iri da kuma kula da yanayin masana'antu kamar yadda muke balaga kafin da bayan sabis na tallace-tallace.Muna son raba ra'ayoyinmu tare da ku kuma muna maraba da sharhi da tambayoyinku.
Bayani
Wannan kit ɗin yana amfani da ginshiƙin jujjuyawar da dabarar da Foregene ya haɓaka, wanda zai iya fitar da tsafta mai inganci da ingancin jimlar RNA daga sel waɗanda aka kirkira a cikin faranti 96, 24, 12, da 6-riji.
Kit ɗin yana ba da ingantacciyar ginshiƙin Tsabtace DNA, wanda zai iya raba mai ƙarfi da sel lysate cikin sauƙi, ɗaure da cire DNA na genomic.Aikin yana da sauƙi kuma yana adana lokaci.
Rukunin RNA-kawai na iya ɗaure RNA da kyau da kyau tare da tsari na musamman.Ana iya sarrafa adadi mai yawa na samfurori lokaci guda.
Abubuwan Kit
| Kayan abun ciki | RE-03111 | RE-03114 |
| 50 T | 200 T | |
| Buffer cRL1* | ml 25 | 100 ml |
| Buffer cRL2 | ml 15 | ml 60 |
| RW1* | ml 25 | 100 ml |
| Farashin RW2 | ml 24 | ml 96 |
| RNase-Free ddH2O | ml 10 | ml 40 |
| Rukunin RNA-Kawai | 50 | 200 |
| Rukunin Tsabtace DNA | 50 | 200 |
| Umarni | 1 | 1 |
Da fatan za a sa safar hannu kuma ku ɗauki matakan kariya yayin aikin kamar yadda Buffer cRL1 da Buffer RW1 ke ɗauke da gishiri masu ban haushi.
Fasaloli & fa'idodi
∎ Dukkanin tsarin ana sarrafa shi a cikin zafin jiki (15-25 ℃), ba tare da wankan kankara da ƙananan zafin jiki ba.
∎ Gabaɗaya kit ɗin ba shi da RNase, babu buƙatar damuwa game da lalata RNA.
■ Rukunin Tsabtace DNA yana ɗaure DNA ta musamman, ta yadda kit ɗin zai iya cire gurɓataccen DNA ba tare da ƙara ƙarin DNA ba.
∎ Yawan yawan amfanin RNA: Rukunin RNA-kawai da dabara na musamman na iya tsarkake RNA da kyau.
n Gudun sauri: mai sauƙin aiki kuma ana iya kammala shi cikin mintuna 11.
∎ Tsaro: Ba a buƙatar reagents na halitta.
∎ Kyakkyawan inganci: RNA da aka tsarkake tana da tsafta, ba ta da furotin da sauran ƙazanta, kuma tana iya saduwa da gwaje-gwaje daban-daban na gaba.
Kit aikace-aikace
Ya dace da hakar da tsarkakewa na jimlar RNA daga sel masu al'ada a cikin faranti 96, 24, 12, da 6-rijiya.
Gudun aiki
zane
Zane-zanen baturi na Agarose Gel na Kit ɗin Warewa na Cell Total RNA ya yi maganin lambobi daban-daban na sel, 20μl elution, ɗauki 2μl tsarkakakkun jimlar RNA 1%.
Adana da rayuwar shiryayye
Ana iya adana kit ɗin na tsawon watanni 12 a zazzabi na ɗaki (15-25 ℃) ko 2-8 ℃ na tsawon lokaci (watanni 24).
Ana iya adana buffer cRL1 a 4 ℃ na wata 1 bayan ƙara 2-hydroxy-1-ethanethiol (na zaɓi) .Kyakkyawan inganci yana zuwa farawa tare da;sabis shine kan gaba;kungiyar ne hadin gwiwa" ne mu sha'anin falsafar da aka akai-akai lura da kuma bi da mu m for High Quality China High Efficiency Manufacturer Bioer Bsc53 Magabio Plus Total Rna tsarkakewa Kit, Mun yi imani da cewa mu dumi da kuma qualified taimako zai kawo muku m surprises da kuma arziki.
Kyakkyawan inganciChina Extraction Reagent, Magnetic Beads, Ta hanyar haɗawa da masana'antu tare da sassan kasuwancin waje, za mu iya ba da jimlar abokin ciniki mafita ta hanyar tabbatar da isar da samfuran da suka dace da kuma mafita zuwa wurin da ya dace a daidai lokacin, wanda ke goyan bayan abubuwan da muke da su da yawa, ƙarfin samarwa mai ƙarfi, daidaiton inganci, kayayyaki iri-iri da kuma kula da yanayin masana'antu kamar yadda muke balaga kafin da bayan sabis na tallace-tallace.Muna son raba ra'ayoyinmu tare da ku kuma muna maraba da sharhi da tambayoyinku.
Ba a fitar da RNA ko amfanin RNA yayi ƙasa
Sau da yawa akwai dalilai iri-iri waɗanda ke shafar ingancin farfadowa, kamar: samfurin nama na RNA abun ciki, hanyar aiki, ƙarar haɓaka, da sauransu.
1. Ice wanka ko cryogenic (4 ° C) centrifugation aka yi a lokacin aiki.
Shawarwari: Yi aiki a dakin da zafin jiki (15-25 ° C) a duk tsawon tsari, kada ku yi wanka da centrifuge a ƙananan yanayin zafi.
2. Ƙimar samfurin da ba daidai ba ko yawan lokacin ajiyar samfurin.
Shawarwari: Ajiye samfurori a -80 ° C ko daskare a cikin ruwa nitrogen kuma kauce wa daskare-narke maimaita amfani;yi ƙoƙarin amfani da sabobin nama ko ƙwayoyin al'ada don hakar RNA.
3. Rashin isasshen samfurin lysis.
Shawarwari: Lokacin da aka haɗa nama, tabbatar da cewa nama ya isa daidai kuma cewa ƙwayoyin nama sun rabu sosai don bayyana sakin RNA.
4. Ba a ƙara eluent daidai ba.
Shawarwari: Tabbatar da cewa RNase-Free ddH2O ana ƙara juzu'i zuwa tsakiyar membrane ginshiƙin tsarkakewa.
5. Ba a ƙara madaidaicin ƙarar cikakken ethanol zuwa Buffer RL2 ko Buffer RW2 ba.
Shawarwari: Bi umarnin, ƙara madaidaicin ƙarar cikakkar ethanol zuwa Buffer RL2 da Buffer RW2 kuma gauraya da kyau kafin amfani da kit ɗin.
6. Nama samfurin sashi bai dace ba.
Shawarwari: Yi amfani da 10-20 MG na nama ko (1-5) × 106Kwayoyin a cikin 500 μl buffer RL1, saboda yawan amfani da nama zai iya haifar da raguwar hakar RNA.
7. Rashin ingantaccen ƙarar ƙyalli ko rashin cikawa.
Shawarwari: Girman haɓakar ginshiƙin tsarkakewa shine 50-200 μl;idan tasirin elution bai gamsar ba, ana ba da shawarar tsawaita lokacin sanyawa dakin zafin jiki bayan ƙara preheated RNase-Free ddH2O, misali na 5-10 min.
8.Shafin tsarkakewa yana da ragowar ethanol bayan wankewar buffer RW2.
Shawarwari: Idan akwai ragowar ethanol bayan wankewar Buffer RW2, centrifugation mara amfani na 1min, lokacin aikin centrifugation na bututu mara kyau za'a iya ƙara zuwa 2min, ko kuma ana iya sanya ginshiƙin tsarkakewa a dakin da zafin jiki na 5 min don cire isasshen ethanol.
RNA mai tsafta ya lalace
Ingancin RNA da aka tsarkake yana da alaƙa da abubuwa kamar adana samfurin, gurɓataccen RNase, da magudi, da sauransu.
1. Ba a adana samfuran nama a cikin lokaci.
Shawarwari: Idan ba a yi amfani da samfurori na nama ko sel a cikin lokaci ba bayan tattarawa, nan da nan a adana a -80 ° C ko nitrogen mai ruwa.Don cire RNA, yi amfani da sabon samfurin nama ko tantanin halitta a duk lokacin da zai yiwu.
2. Maimaita daskare-narke samfuran nama.
Shawarwari: Lokacin adana samfuran nama, yana da kyau a yanke su kanana don adanawa, kuma a cire ɗaya daga cikin guntun lokacin amfani da su don guje wa daskare-narke samfurin da kuma lalata RNA.
3. Ana gabatar da RNase ko a'a sanye da safar hannu, abin rufe fuska, da sauransu yayin aikin.
Shawarwari: Gwajin cirewar RNA an fi yin su a cikin ɗakunan sarrafa RNA daban kuma an share teburin kafin gwajin.
Sanya safar hannu da abin rufe fuska yayin gwajin don rage lalata RNA wanda ya haifar da gabatarwar RNase.
4. Reagents sun gurbata da RNase yayin amfani.
Shawarwari: Sauya da sabon Kit ɗin Keɓewar Dabbobi na RNA don gwaje-gwaje masu alaƙa.
5. Bututun centrifuge, tukwici, da sauransu da ake amfani da su wajen sarrafa RNA sun gurɓata da RNase.
Shawarwari: Tabbatar da cewa bututun centrifuge, tukwici, pipettes, da sauransu. da ake amfani da su wajen cire RNA duk ba su da RNase.
RNA da aka tsarkake yana shafar gwaje-gwaje na ƙasa
RNA da aka tsarkake ta hanyar ginshiƙin tsarkakewa, idan ions gishiri, abun cikin furotin ya yi girma zai shafi gwajin ƙasa, kamar: juzu'i na baya, Northern Blot et al.
1. RNA da aka elutioned yana da ragowar ion gishiri.
Shawarwari: Tabbatar da cewa an ƙara madaidaicin ƙarar ethanol zuwa Buffer RW2 kuma kuyi ginshiƙan tsarkakewa guda 2 a saurin centrifugal da aka nuna don aiki;idan akwai ragowar ion gishiri, bar ginshiƙin tsarkakewa zuwa Buffer RW2 na 5 min a zafin daki kuma yi centrifugation don haɓaka kawar da gurɓataccen gishiri.
2. Ethanol saura a cikin elutioned RNA.
Shawarwari: Tabbatar da cewa bayan buffer RW2 wanka, yi aikin centrifugation mara amfani a cikin saurin centrifugation da aka nuna don aiki, ƙara lokacin aikin centrifugation mara amfani zuwa 2 min idan har yanzu akwai sauran ragowar ethanol, ko barin shi a cikin zafin jiki na 5 m.
