China Maƙeran don 2× Mahimmanci Premix don Samfuran da Ba a Tsaftace Tsabtace Mahimmanci na PCR kai tsaye na Qpcr Mix Plus U
The kungiyar kiyaye a kan hanya manufar "kimiyya management, high quality da kuma yadda ya dace primacy, buyer koli ga kasar Sin Manufacturer for 2 × mayar da hankali Premix for Unpurified Samfurin Real-Time Quantitative PCR Direct Multiplex Probe Qpcr Mix Plus U, Our kasuwanci ne sadaukar domin ba abokan ciniki tare da gagarumin da kuma amintacce saman ingancin kayayyakin a m farashin, faranta wa abokan ciniki da kowane abokin ciniki farashin.
Kungiyar ta ci gaba da aiwatar da manufar "Gudanar da kimiyya, babban inganci da ingantaccen inganci, mafi girman mai siye donChina Taq DNA Polymerase da Qpcr, Kamfaninmu yana da ƙarfi mai yawa kuma yana da tsayayyen tsarin cibiyar sadarwar tallace-tallace.Muna fata za mu iya kulla kyakkyawar dangantakar kasuwanci tare da duk abokan ciniki daga gida da waje bisa ga fa'idar juna.
Ka'idodin ƙira na ainihin lokacin PCR
Gaba Farawa da Juya Farko
Don Real Time PCR, ƙirar farko tana da mahimmanci.Abubuwan haɓaka suna da alaƙa da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙimar PCR, kuma ana iya tsara su tare da la'akari da ƙa'idodi masu zuwa:
- Tsayin farko: 18-30bp.
- GC abun ciki: 40-60%.
- Ƙimar Tm: Software na ƙira na farko, kamar Primer 5, na iya ba da ƙimar Tm na firamare.Ma'auni na Tm na sama da na ƙasa ya kamata su kasance kusa da iyawa.Hakanan ana iya amfani da dabarar lissafin Tm: Tm = 4 °C (G + C) + 2 °C (A + T).Lokacin yin PCR, ana zaɓar zafin da ke ƙasa da ƙimar ƙimar Tm na 5 ° C gabaɗaya azaman zazzabi mai ɗaukar nauyi (madaidaicin haɓakar zafin jiki na iya ƙara ƙayyadaddun halayen PCR).
- Alamar farko da samfuran PCR:
- Tsawon samfurin PCR na ƙirar ƙira ya fi dacewa 100-150bp.
- Ya kamata a kauce wa ƙera ƙirar ƙira a cikin yanki na biyu na samfuri gwargwadon yiwuwa.
- Guji samuwar tushe guda 2 ko sama da haka tsakanin 3′ na ƙofofin sama da na ƙasa.
- Babban tushe 3′ na tashar ba zai iya kasancewa tare da ƙarin ƙarin 3 a jere G ko C.
- Masu farawa da kansu ba za su iya samun ƙarin tsari ba, in ba haka ba za a kafa tsarin gashin gashi, yana shafar haɓaka PCR.
- Yakamata a rarraba ATCG daidai gwargwado kamar yadda zai yiwu a cikin jeri na farko, kuma ya kamata a guje wa tushe na 3′ a matsayin T.
Karin bayani1:Cda DirectRT-qPCR Kit component kari kunshin
1.Cell Lysis Magani
Cell Lysis Magani | |||
Abubuwan Kit (24-riji tsarin lysis / rijiyar) | Saukewa: DRT-01011-A1 | Saukewa: DRT-01011-A2 | |
100 T | 500 T | ||
SasheI | Buffer CL | 20 ml | 100 ml |
Foregene Protease Plus II | 400 ml | 1 ml × 2 | |
Buffer ST | 1 ml × 2 | 10 ml | |
SasheII | Goge DNA | 400 ml | 1 ml × 2 |
RT Mix | |
Abubuwan Kit (20 μl tsarin amsawa) | Saukewa: DRT-01011-B1 |
200 T | |
5× Direct RT Mix | 800 ml |
RNase-Free ddH2O | 1.7 ml × 2 |
qPCR Mix | ||
Abubuwan Kit (20 μl tsarin amsawa) | Saukewa: DRT-01021-C1 | Saukewa: DRT-01021-C2 |
200 T | 1000 T | |
2× Direct qPCR Mix-Taqman | 1 ml × 2 | 1.7 ml × 6 |
20× ROX Reference Dye | 40ml ku | 200 μl |
RNase-Free ddH2O | 1.7 ml | 10 ml |
The kungiyar kiyaye a kan hanya manufar "kimiyya management, high quality da kuma yadda ya dace primacy, buyer koli ga kasar Sin Manufacturer for 2 × mayar da hankali Premix for Unpurified Samfurin Real-Time Quantitative PCR Direct Multiplex Probe Qpcr Mix Plus U, Our kasuwanci ne sadaukar domin ba abokan ciniki tare da gagarumin da kuma amintacce saman ingancin kayayyakin a m farashin, faranta wa abokan ciniki da kowane abokin ciniki farashin.
China Manufacturer donChina Taq DNA Polymerase da Qpcr, Kamfaninmu yana da ƙarfi mai yawa kuma yana da tsayayyen tsarin cibiyar sadarwar tallace-tallace.Muna fata za mu iya kulla kyakkyawar dangantakar kasuwanci tare da duk abokan ciniki daga gida da waje bisa ga fa'idar juna.
Ka'idodin ƙira na ainihin lokacin PCR
Gaba Farawa da Juya Farko
Don Real Time PCR, ƙirar farko tana da mahimmanci.Abubuwan haɓaka suna da alaƙa da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙimar PCR, kuma ana iya tsara su tare da la'akari da ƙa'idodi masu zuwa:
- Tsayin farko: 18-30bp.
- GC abun ciki: 40-60%.
- Ƙimar Tm: Software na ƙira na farko, kamar Primer 5, na iya ba da ƙimar Tm na firamare.Ma'auni na Tm na sama da na ƙasa ya kamata su kasance kusa da iyawa.Hakanan ana iya amfani da dabarar lissafin Tm: Tm = 4 °C (G + C) + 2 °C (A + T).Lokacin yin PCR, ana zaɓar zafin da ke ƙasa da ƙimar ƙimar Tm na 5 ° C gabaɗaya azaman zazzabi mai ɗaukar nauyi (madaidaicin haɓakar zafin jiki na iya ƙara ƙayyadaddun halayen PCR).
- Alamar farko da samfuran PCR:
- Tsawon samfurin PCR na ƙirar ƙira ya fi dacewa 100-150bp.
- Ya kamata a kauce wa ƙera ƙirar ƙira a cikin yanki na biyu na samfuri gwargwadon yiwuwa.
- Guji samuwar tushe guda 2 ko sama da haka tsakanin 3′ na ƙofofin sama da na ƙasa.
- Babban tushe 3′ na tashar ba zai iya kasancewa tare da ƙarin ƙarin 3 a jere G ko C.
- Masu farawa da kansu ba za su iya samun ƙarin tsari ba, in ba haka ba za a kafa tsarin gashin gashi, yana shafar haɓaka PCR.
- Yakamata a rarraba ATCG daidai gwargwado kamar yadda zai yiwu a cikin jeri na farko, kuma ya kamata a guje wa tushe na 3′ a matsayin T.
Karin bayani1:Cda DirectRT-qPCR Kit component kari kunshin
1.Cell Lysis Magani
Cell Lysis Magani | |||
Abubuwan Kit (24-riji tsarin lysis / rijiyar) | Saukewa: DRT-01011-A1 | Saukewa: DRT-01011-A2 | |
100 T | 500 T | ||
SasheI | Buffer CL | 20 ml | 100 ml |
Foregene Protease Plus II | 400 ml | 1 ml × 2 | |
Buffer ST | 1 ml × 2 | 10 ml | |
SasheII | Goge DNA | 400 ml | 1 ml × 2 |
RT Mix | |
Abubuwan Kit (20 μl tsarin amsawa) | Saukewa: DRT-01011-B1 |
200 T | |
5× Direct RT Mix | 800 ml |
RNase-Free ddH2O | 1.7 ml × 2 |
qPCR Mix | ||
Abubuwan Kit (20 μl tsarin amsawa) | Saukewa: DRT-01021-C1 | Saukewa: DRT-01021-C2 |
200 T | 1000 T | |
2× Direct qPCR Mix-Taqman | 1 ml × 2 | 1.7 ml × 6 |
20× ROX Reference Dye | 40ml ku | 200 μl |
RNase-Free ddH2O | 1.7 ml | 10 ml |
Jagoran Jagora: