Gabatarwa:

Kwayoyin RNA na iya yin mu'amala da yawa tare da sauran kwayoyin halitta, kamar furotin, DNA da RNA, don haka suna wanzuwa a cikin sel da kwayoyin halitta a cikin sigar macromolecular complexes.Daga cikin su, RNA-protein complexes sune wanzuwa da aikin RNA Ɗaya daga cikin mahimman siffofin.Rushewar RNA wata hanya ce ta yau da kullun don tantancewa da gano ƙwayoyin RNA, musamman ma'amalar sunadaran kwayoyin lncRNA, kuma galibin masu bincike da mujallun ilimi na duniya sun gane su.A yau FORREGENE zai raba tare da ku ƙa'idar gwaji da tsarin fasaha na cirewar RNA, watakila za ku iya amfani da shi.

1. Ƙa'idar gwaji ta RNA ta rushe fasaha:

Rubutun in vitro da haɗin biotin-labeled manufa lncRNA ko lncRNA gutsuttsura (ciki har da gutsuttsarin RNA da aka yi amfani da su azaman ingantattun sarrafawa da korau), da kuma haɗawa tare da tsantsar sel;yi amfani da avidin-anchored magnetic beads don tattara RNA-protein ko RNA-RNA complexes, Bayan wankewa sosai, hadaddun ya ɓace.Idan gano kwayoyin manufa na sunadaran da ke hulɗa da lncRNA, yi electrophoresis na furotin akan samfurin RNA-jawo ƙasa, gano furotin da aka yi niyya ta hanyar tabon azurfa, kuma a yanke gel don ƙididdigar taro;Hakanan kai tsaye yin ƙwanƙwasa ƙoƙon yamma akan samfurin da aka cire;Idan an gano Don kwayoyin manufa na RNA waɗanda ke yin hulɗa da lncRNA, samfuran RNA-jawo suna ƙarƙashin cirewar RNA kuma ana yin takamaiman gano qRT-PCR.

2. RNA ta rushe tsarin gwajin fasaha:

3. Sabis na fasaha don RNA ya rushe FOREGENE:

Al'adar salula

Shiri na jimlar tantanin halitta/nukiliya/matsayin furotin plasma

RNA ja saukar gwaji

SDS-PAGE electrophoresis, azurfa tabo

Mass spectrometry da gwajin WB

Rahoton gwaji

4. Shari'ar gwaji

Hoton da ke gaba yana nuna sakamakon FJ Bio-Bio's RNA runguma da gano SDS-PAGE electrophoresis, wanda a cikinsa kibiya ja ke nuna alamar bambancin band:

Rukunin bincike na Farfesa Song Xu na Jami'ar Sichuan ya tattara shekaru 15 na dogon lokaci a fagen bincike na lncRNA.Foregene Bio da ƙungiyar bincike na Farfesa Song Xu sun haɗa kai don ƙaddamar da fasahar RNA ja na lncRNA, wanda zai ba ku sakamako mai gamsarwa don gwajin ku.