Game da hakar RNA

Shekaru talatin da suka gabata, don hakar RNA, abin da zamu iya tunani shine kawai yadda ake samun cikakken RNA, duk da haka, babu buƙatu don saurin cirewa (Fasahar ƙarni na farko da Hanyar Trizol da aka Haifa).Tare da sabuntawar sabuntawar fasahar hakar RNA, abin da za mu iya yi bai iyakance ga samun sakamako ba.A lokacin da fuskantar hakar na farko-ƙarni na tushen reagents Trizol, experimenters kasance gaba ɗaya dame da m aiki matakai;ko da yake bayan yawancin ingantawa da sababbin abubuwa, har yanzu ya ɗauki lokaci mai yawa.A lokaci guda, ana buƙatar amfani da reagents na halitta kamar phenol da chloroform a cikin aikin hakar, wanda kuma ke yin barazana ga amincin ma'aikatan da suka dace.

Inganci, dacewa da aminci

Nemo ingantaccen, dacewa kuma amintaccen samfurin hakar RNA ya zama babban burin masu gwaji.Don haka, hanyar tallan Silica Spin Column don hakar RNA ta kasance.Kayayyakin hakar RNA na ƙarni na uku na Foregene sun zana fa'idodin samfuran haƙon ginshiƙi na baya, ta amfani da (DNA-Cleaning + RNA kawai) fasahar Spin Column guda biyu don cire gDNA da tarkacen nama a lokaci guda, ba tare da maganin DNA na ragowar gDNA ba, kuma ba a ƙara amfani da phenol, da sauransu. Guba da cutarwa ga ƙarancin ƙarancin lokaci, don samun ƙarancin ƙarancin lokaci, da rashin ƙarfi na RNA.

ginshiƙi kwatanta tsarin aiki

Jadawalin kwatancen sakamakon gwaji

Foregene jimlar keɓancewar RNA yana da babban yawan haɓakar hakar RNA, tsafta mai kyau, kuma tasirin hakar miRNA ya fi sauran kayan aikin alama

Electrophoresis na 50 MG na hanta linzamin kwamfuta duka RNA da aka fitar ta hanyoyi daban-daban
Zamanin farko (1: Trizol)
Ƙarni na biyu (2: RNA kayan cirewa daga kamfanin A)
Ƙarni na uku (3: kayan cirewar RNA da na kamfanin A, 4: Foregene: RNA keɓe kayan)

qPCR jadawali (girma mai lankwasa jadawali)

Dole ne a cire DNA na genomic yadda ya kamata yayin hakar RNA, in ba haka ba zai sami tasirin da ba za a iya sarrafawa ba akan gwaje-gwajen ƙasa (ƙimar CT ta farko, tabbataccen ƙarya).Kit ɗin na gaba na Foregene na iya cire DNA ɗin daidai ba tare da ƙari na DNA ba, yana adana lokaci mai yawa, tare da guje wa lalacewa ta hanyar gurɓatawar RNase na waje.
ƙarni na farko (kore yana nuna TRlzol-, ja yana nuna TRlzol +)

Ƙarni na biyu (Blue yana nuna Kamfanin A RNA kayan hakar -, ja yana nuna Kamfanin A RNA kayan hakar +)

Ƙarni na uku (blue yana nuna kamfani A RNA kayan cirewa da ƙari, kore yana nuna Foregene RNA keɓe kayan aiki)

(Lura: "_" a cikin jadawali na qPCR yana nuna cewa ba a ƙara DNA ba, kuma ba a cire gurɓataccen DNA ba; "+" yana nuna cewa an ƙara DNA, kuma an cire gurɓataccen DNA)

Cikakkun hakar RNA a cikin mintuna 11

Babu buƙatar shekaru na tarin abubuwan haɓakar haɓakar acid nucleic, Foregene babban inganci jimlar samfuran keɓancewar RNA na iya ba ku damar samun babban amfanin ƙasa da RNA mai tsabta a cikin mintuna 11 don saduwa da buƙatun gwaji iri-iri.

Ya zuwa yanzu, Foregene jimlar RNA isolaton jerin samfuran samfuran ɗimbin abokan ciniki sun san su kuma sun ba da gudummawa ga wallafe-wallafe masu yawa.Ainihin yadda abokin ciniki ya yi amfani da taswirar ra'ayi na electrophoresis da ma'aunin haɓakar PCR mai ƙididdigewa bayan jujjuya rubutun ya nuna cewa RNA da aka fitar ta amfani da samfuran Foregene ba wai kawai mai girma bane a cikin ƙima, amma kuma cikin kyakkyawan mutunci.Fasahar samfur da aka gwada ta kasuwa ne kawai kowa zai iya fifita shi.

Salon Warewa Kit ɗin RNA

Babban yawan amfanin RNA

Mai sauri: Gama Warewar RNA a cikin mintuna 11

Tsaro: Babu wani sinadari na halitta da aka ƙara

Wasu nassoshi masu girma:

1. Yuan Fang, Zezhong Liu, Yang Qiu, da dai sauransu.Hana mai hana ƙwayoyin cuta na ƙwayoyin cuta na RNAi ta hanyar peptides mai zane yana kare kariya daga kamuwa da cuta ta enteroviral a cikin vivo, Immunity, 54(10), 2021: 2231-2244.e6.(IF: 31.745,Kit ɗin keɓewar RNA na hotoCat No.RE-02011)

https://www.sciencedirect.com/science/article/pii/S1074761321003617

2. Ren, Y., Wang, A., Wu, D. et al.Hani biyu na rigakafi na asali da apoptosis ta hanyar furotin cytomegalovirus ɗan adam UL37x1 yana ba da damar kwafin ƙwayar cuta mai inganci.Nat Microbiol 7, 1041-1053 (2022).(IF: 30.964,Salon Warewa Kit ɗin RNACat No.RE-03111)

doi.org/10.1038/s41564-022-01136-6