Nawa ka sani

Game da Nucleic acid hakar

Farko da ƙarshen tsaftataccen acid nucleic

Komai yana da wahala a farkon, tsabtace nucleic acid shine mafi

Ƙaddamar da gwaje-gwajen kwayoyin halitta, don gwaje-gwaje na gaba

Nasara ko gazawa yana da tasiri mai mahimmanci.

Trace/ultra-trace UV spectrophotometer yana da fifiko daga yawancin masu binciken kimiyya saboda yana iya gano ƙwayar nucleic acid da ragowar furotin da gishiri a sauƙaƙe, cikin sauri da tattalin arziki.

Kamar yadda muka sani, A260 yana nufin nucleic acid absorbance OD darajar, A280 yana nufin furotin maida hankali absorbance OD darajar, A230 yana nufin gishiri ion maida hankali absorbance OD darajar, don haka A260 iya maida nucleic acid maida hankali, A260/280 yana nufin gina jiki saura, A260/230 yana nufin gishiri ion ragowar, amma wannan shi ne babban sakamakon binciken da aka gano a kan babban sakamakon bincike.na al'ada"don yarda cewa zai iya bayyana tsarkin DNA da RNA zuwawani iyaka.Ba shine kawai ma'auni don gano yawan amfanin acid na nucleic da tsarki ba, ana amfani da shi kawai azaman tunani, kuma ba shine "ma'auni na zinariya".

Littafin Thermo Fisher ND-2000 yana jaddada amincin sakamakon gwajin

Dalilin shi ne cewa sakamakon da aka samu ta hanyar amfani da micro / ultramicro UV spectrophotometer kawai yana nuna yawan amfanin ƙasa da tsarki na nucleic acid daga gefe, kuma akwai abubuwa da yawa masu tasiri (hanyar gani, samfurin samfurin, ƙaddamarwar samfurin, ƙimar pH, sauran ions da ke shafar ma'auni, da dai sauransu) , ƙimar OD da aka auna ba lallai ba ne daidai.A lokaci guda kuma, saboda ƙarancin ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙimar ƙimar abin sha, DNA mai ɗaci ɗaya, DNA mai ɗaure biyu, RNA, dNTPs, da wasu sunadaran duk suna da kololuwar ɗaukar nauyi a tsayin 260nm, kuma ƙaddamarwar da A260 ya ƙaddara shi kaɗai ba lallai ba ne ainihin DNA ko RNA.Tattaunawa, da mutuncinsa da lalacewarsa ba za a iya tantance shi ba.

To ta yaya za a yi hukunci da ingancin tsaftataccen acid nucleic mu?Baya ga yin amfani da micro/ultramicro UV spectrophotometer don ganowa, ana kuma buƙatar hanyoyin kamar agarose gel electrophoresis don nunawa a gani da tsarki da amincin acid ɗin nucleic, kuma don nuna ƙaddamarwa zuwa wani ɗan lokaci.Ta wannan hanyar, yawan amfanin acid nucleic da tsabta da aka samu daga sakamakon gano hanyoyin daban-daban suna da gaskiya.

Kwatancen ginshiƙi na gwaji

An fitar da DNA na kwayoyin halitta na kwayoyin H1299 ta hanyar amfani da kayan aikin DNA na kamfanin A, kuma an ga ƙazanta mai mahimmanci, wanda ya haifar da ƙimar OD mai girma.

(ƙimar auna OD da madaidaicin electropherogram)

Fihirisar kimantawa

Shin akwai "ma'auni na zinariya" don yawan amfanin acid nucleic da gwajin inganci?

Kamar yadda muka sani, babu wata hanya mai sauƙi, sauri da arha.Ko yana da spectrophotometer, gel electrophoresis, ko mass spectrometry, duk suna nuna ƙaddamarwa da tsabta na nucleic acid a cikin bayani daga bangarori daban-daban, kuma suna buƙatar yin hukunci ta hanyar magana da juna.

Mafi kyawun ma'aunin ƙima koyausheaikace-aikacen gwaji na biyo baya.Ba ya shafar ingancin jujjuya rubutu da haɓaka PCR.Samun ingantattun samfuran haɓakawa da ƙimar Ct, da samun cikakken jeri ta hanyar jeri shine nasarar hako acid nucleic.

Don taƙaitawa, ra'ayi na ka'idar rabo-kawai ya kamata a ƙalubalanci ta hanyar ra'ayi na "amfani da kyakkyawan sakamakon gwaji na ƙasa a matsayin ma'auni mai kyau na cirewa"!

Abubuwan da aka ba da shawarar Foregene DNA RNA kayan haɓaka don samun babban tsaftar DNA/RNA:

https://www.foreivd.com/reagent/dna-isolation-series/

https://www.foreivd.com/reagent/rna-isolation-series/