Ana amfani da enzyme Taq mai zafi sosai.Idan aka kwatanta da DNA polymerase na yau da kullun, zafin farawa Taq enzyme zai iya guje wa wasu ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun abubuwa da samuwar dimers na farko, kuma yana iya haɓaka ƙimar ƙimar haɓakar ƙwayoyin cuta yadda yakamata.Musamman a fagen gwajin kwayoyin halitta, an gano taq enzyme mai zafi a matsayin ma'auni na wajibi a cikin masana'antar, kuma bai kamata a yi amfani da DNA polymerase na yau da kullun ba.Kamar yadda ake iya gani daga sama, ana amfani da enzymes na Taq mai zafi.A halin yanzu, akwai nau'ikan nau'ikan enzymes na Taq masu zafi a kasuwannin cikin gida, amma babu yawancin enzymes na Taq masu zafi masu inganci.Fuskantar samfuran kayan enzyme Taq masu zafi da yawa, ta yaya za mu zaɓa?

1. Zaɓi enzyme na Taq mai zafi tare da ingantaccen haɓakawa

Ingantaccen haɓakawa na PCR yana da alaƙa da aikin Taq enzyme.Bayan ingantaccen tsarin amsawar enzyme na Taq mai kyau, ingancin haɓaka yana sama da 95%, kuma kewayon haɓakawa na adadin samfurin farko yana da faɗi.Za a iya samun haɓaka mai gamsarwa lokacin da abin da ake nufi da abubuwan da aka yi niyya ya yi ƙasa, kuma ba shi da sauƙi a sha guba lokacin da adadin samfuri ya yi yawa, kuma lokacin ƙarawa ya daɗe.Don Taq enzyme tare da rashin aiki mara kyau, ko da an inganta tsarin amsawa sau da yawa, haɓakar haɓakawa har yanzu bai wuce 90% ba, siffar "S" na ma'auni na haɓakawa ba a bayyane yake ba, gangaren yana da ƙananan, kuma kullun yana da lebur.Lokacin da adadin samfuri ya yi ƙasa, ba za a iya ƙarawa ba, kuma lokacin da adadin samfuri ya yi girma, tasirin haɓakawa bai dace ba.Don haka, zaɓin polymerases na DNA tare da ingantaccen haɓakawa yana da mahimmanci ga nasarar PCR da qPCR.

2. Zaɓi enzyme Taq mai zafi mai zafi tare da ƙarfin enzyme mai ƙarfi

Ikon enzymatic na Taq enzyme yana da alaƙa da haɓakar haɓakawa.Gabaɗaya, ƙarfin ƙarfin enzymatic na Taq enzyme mai zafi, mafi tsayin lokacin girma na haɓakawa na PCR, mafi yawan yanayin 'S-shaped', mafi girman ƙimar siginar walƙiya, kuma mafi dacewa da gano PCR mai yawa.Alamar DNA polymerases tare da raunin enzymatic ikon gabaɗaya na iya tallafawa halayen 2-plex kawai.Lokacin yin halayen 3-plex, madaidaicin ƙararrawa yana da ƙasa, ƙimar siginar walƙiya ta yi ƙasa, kuma babu yanayin haɓakawa na yau da kullun, don haka sakamakon yana da wahala a yanke hukunci.

3. Zaɓi enzyme Taq mai zafi mai zafi tare da babban hankali

Gabaɗaya magana, DNA polymerase yana da ingantaccen haɓakawa da haɓakawa mai girma, amma kuma akwai rashin daidaituwa.Idan maƙasudin ƙayyadaddun ƙayyadaddun ƙirar samfurin da za a ƙarawa ya yi ƙasa, ana ba da shawarar gwada haɓakar haɓakar enzyme Taq.Hanyar ganowa ta yau da kullun ita ce aiwatar da dilution mai ninki 10 ko ninki 5 na ɓarkewar ƙwayar ƙwayar ƙwayar cuta, aiwatar da gano PCR a ƙaramin dilution, sannan zaɓi enzyme Taq mai zafi tare da ƙwarewar ganowa mafi girma.

Ana iya gani daga abubuwan da ke sama cewa masu bincike suna buƙatar zaɓar bisa ga buƙatun gwajin su da yanayin kuɗi.Zai fi kyau a yi gwajin haɓakar haɓakar gradient don gano ingancin haɓakawa da azanci na enzyme Taq mai zafi.

Misali na Foregene'Taq DNA Polymerase:

Foreasy HS Taq DNA Polymerase

Bayani

Foreasy HS Taq DNA Polymerase shine DNA polymerase wanda aka bayyana a cikin ƙwayoyin injiniya na Escherichia coli ta hanyar fasahar sake haɗawa.Ana haɗe enzyme tare da buffffer na musamman, wanda ke sa samfurin ya zama mai juriya da jituwa, kuma yana iya amfani da samfurin lysate kai tsaye (tsarin Foregene Lysis) azaman samfuri don halayen ganowa.

Aikace-aikace

Ingantacciyar PCR da gano PCR masu ƙididdigewa na samfuran tsarkakakku da samfuran da ba a tsarkake ba.

Kula da inganci

1.Ba a gano ayyukan nuclease na waje ba

Hanyar 2.PCR don gano ragowar genomic DNA ba tare da mai watsa shiri ba

3. Yana iya inganta haɓakar kwayoyin halitta guda ɗaya a cikin Genome na ɗan adam

4.Ajiye a dakin da zafin jiki na mako guda, babu shakka canje-canjen ayyuka

Bayanin samfur: https://www.foreivd.com/foreasy-hs-taq-dna-polymerase-product/