Kafa gwajin SOP na PCR don daidaita halayen ma'aikatan gwaji.
Gwajin yana mutuƙar bin hanyoyin aiki, kuma yana rage gurɓataccen gurɓataccen PCR wanda zai iya haifar da abubuwan ɗan adam ko kuma ya hana faruwar ƙazanta.Bugu da ƙari, mai gwaji ya kamata ya sami daidaitattun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararru, gami da ƙwarewar aiki da kayan aikin da ke da alaƙa, fayyace duk tsarin aikin, ƙware hanyoyin jiyya na gurɓatawa da hanyoyin kula da ingancin dakin gwaje-gwaje, da samun damar fassara sakamakon gwajin daidai.
Kafa madaidaicin dakin gwaje-gwaje na PCR.
dakin gwaje-gwaje PCR ya kasu kashi hudu bisa ka'ida, wato yankin shirye-shiryen reagent, yankin sarrafa samfurin, yanki na haɓakawa, da yankin nazarin samfuran haɓakawa.Wuraren biyu na farko su ne wuraren haɓakawa, kuma yankuna biyu na ƙarshe sune wuraren haɓakawa.Ya kamata a raba yankin pre-amplification da yankin bayan haɓakawa sosai.Gwaji kayan, reagents, rikodi takarda, alkalama, tsaftacewa kayan, da dai sauransu, iya kawai gudana daga pre-amplification yankin zuwa post-amplification yankin, wato, daga reagent yankin shirye-shirye → samfurin sarrafa yankin → ƙarawa yankin → amplification samfurin analysis yankin, kuma dole ne ba gudu a baya .Hakanan ya kamata iskar da ke cikin dakin gwaje-gwaje ta gudana daga wurin da aka riga aka yi amfani da su zuwa yankin bayan karawa, kuma kada ta koma baya.An nuna kyakkyawan ƙirar dakin gwaje-gwaje na PCR a ƙasa:
Hoto A: Kyakkyawan yanayin saitin dakin gwaje-gwaje na PCR tare da matsi mara kyau a cikin dakin ma'auni
Hoto B: Madaidaicin yanayin saitin dakin gwaje-gwaje na PCR tare da matsi mai kyau a cikin dakin ma'auni
Zane-zanen saitin dakin gwaje-gwaje na PCR da aka bayar a cikin Hoto A da Hoto B yakamata ya zama mafi kyawun yanayin saitin, kuma dakin gwaje-gwaje tare da yanayi na iya komawa zuwa wannan yanayin don ƙira.Don dakunan gwaje-gwaje na yau da kullun, ana ba da shawarar cewa za a iya raba yankin haɓakawa na PCR da yankin nazarin samfuran, kuma buɗe murfin ya kamata a rage gwargwadon yiwuwa a yankin shirye-shiryen samfurin da yankin haɓakawa na PCR.Tuna: Kayayyaki da kayan gwaji a cikin yankin binciken samfur an hana su kai tsaye zuwa yankin shirye-shiryen samfurin da yankin haɓaka PCR.
Idan dakin gwaje-gwaje yana yin ganowa da gano PCR kawai, ana ba da shawarar yin amfani da PCR mai kyalli maimakon PCR na al'ada.
Za'a iya tattara sakamakon gano ƙimar PCR na Fluorescence da bincika ta siginar kyalli, don haka babu buƙatar buɗe murfin don electrophoresis bayan abin da ya faru, wanda ke guje wa gurɓataccen samfuran PCR wanda ke haifar da zubar da samfuran dauki don samar da iska.Idan ka ƙara yawan buɗewar hula yayin ɗaukar nauyin gel electrophoresis, ana iya samun gurɓataccen iska.Ana ba da shawarar haɓaka aikace-aikacen PCR mai ƙididdigewa kuma a hankali maye gurbin PCR mai inganci.
Ana amfani da tsarin gurɓataccen samfur na anti-PCR don amsawar PCR.
Tsarin yana amfani da dUTP maimakon dTTP.Bayan amsawar PCR, duk samfuran PCR (gutsiyar DNA) an haɗa su tare da dUTP;a zagaye na gaba na amsawar PCR, UNG enzyme da aka ƙara a cikin tsarin ana sanya shi a 37 ° C na minti 5 kafin PCR, wanda zai iya lalata duk sassan DNA da ke dauke da dUTP, sa'an nan kuma yin aikin PCR.Wannan na iya cire gaba ɗaya gurɓatar iska da samfuran PCR ke haifarwa.Ana nuna tasirin a cikin hoton da ke ƙasa:
Lura: Don jerin PCR kai tsaye, zaku iya zaɓar samfuran samfuran tsarin lalata samfuran anti-PCR na Foregene.Ba da shawara
Don dakunan gwaje-gwajen da ke gudanar da gwaje-gwaje masu girma na genotyping, ana ba da shawarar sosai a yi amfani da tsarin gurɓataccen samfur na UNG anti-PCR don gwajin sakewa baya ga gina dakunan gwaje-gwaje masu ma'ana.
Tunatarwa: Amfani da wannan tsarin ba zai iya cire gurɓatar samfurin PCR da aka rigaya ya haifar ba.Don haka, yakamata a yi amfani da tsarin UNG a farkon gwajin da ya dace, kuma yakamata a yi amfani da tsarin UNG don haɓaka PCR, don hana gurɓatar samfuran PCR.
Ana ba da shawarar yin amfani da tsarin PCR-UNG kai tsaye na Foregene yayin gudanar da babban gwaji, kamar:
Ganyen Shuka Kai tsaye PCR Kit-UNG;
Kayan Shuka Kai tsaye PCR Kit-UNG;
Kayan Naman Dabbobi Kai tsaye PCR Kit-UNG;
Mouse Tail Direct PCR Kit-UNG;
Kifi na Zebra Direct PCR Kit-UNG.
Wannan jerin kits daga Foregeneba zai iya aiwatar da gano PCR cikin sauri da kuma a kan babban sikeli ba, amma kuma yana hanawa da sarrafa gurɓatar samfuran PCR yadda ya kamata.
Lokacin aikawa: Maris 19-2021
