Wanda aka buga: CAS Key Laboratory na Pathogen Microbiology and Immunology, Cibiyar Nazarin Kwayoyin Halitta, Kwalejin Kimiyya ta kasar Sin
Saukewa: 69.504
Samfurin Talla:Salon Warewa Kit ɗin RNA
Asalin mahada:https://www.nature.com/articles/s41586-022-05271-2
Cututtuka da filoviruses kamar cutar Ebola (EBOV) da cutar Marburg na iya haifar da mummunan alamun asibiti, gami da zazzabin jini da gazawar ƙwayoyin cuta.4.Anan mun ƙaddara tsarin ƙwayar cutar Ebola L protein a cikin hadaddun tare da tetrameric VP35 ta amfani da microscopy-electron microscopy (jihar 1).Binciken tsarin ya nuna cewa cutar Ebola ta L tana da wani nau'in shigar da takamaiman filovirus wanda ke da mahimmanci don haɗin RNA, kuma VP35 yana hulɗa da yawa tare da yankin N-terminal na L ta hanyar protomers uku na tetramer VP35.
A cikin wannan takarda, an fitar da jimlar RNA ta amfani da ForegeneSalon Warewa Kit ɗin RNAkuma Nanodrop ya auna ƙarfin RNA a 260 nm, sannan a yi gano RT/qPCR.
∎ Dukkanin tsarin ana sarrafa shi a cikin zafin jiki (15-25 ℃), ba tare da wankan kankara da ƙananan zafin jiki ba.
∎ Gabaɗaya kit ɗin ba shi da RNase, babu buƙatar damuwa game da lalata RNA.
■ Rukunin Tsabtace DNA yana ɗaure DNA ta musamman, ta yadda kit ɗin zai iya cire gurɓataccen DNA ba tare da ƙara ƙarin DNA ba.
∎ Yawan yawan amfanin RNA: Rukunin RNA-kawai da dabara na musamman na iya tsarkake RNA da kyau.
n Gudun sauri: mai sauƙin aiki kuma ana iya kammala shi cikin mintuna 11.
∎ Tsaro: Ba a buƙatar reagents na halitta.
∎ Kyakkyawan inganci: RNA da aka tsarkake tana da tsafta, ba ta da furotin da sauran ƙazanta, kuma tana iya saduwa da gwaje-gwaje daban-daban na gaba.
Lokacin aikawa: Nuwamba-04-2022
