Fiye da rabin 2022, zo ku ga abin da manyan labaran da Foeregene ya buga!
01
Idan: 17.694
An buga ta: Asibitin Haɗin Kai na Farko, Jami'ar Sun Yat-sen
Babban abun ciki: Esophageal squamous cell carcinoma (ESCC) shine babban nau'in ciwon daji na esophageal.Gano ingantattun maƙasudin warkewa yana da matuƙar mahimmanci don ingantacciyar jiyya ta ESCC.Wasu nazarin sun nuna cewa rashin kuskuren RNA na iya haɓaka magana da fassarar mRNAs masu alaƙa da ƙwayoyin kansa, amma tsarin ƙwayoyin cuta ya kasance ba a sani ba.Wannan binciken ya nuna cewa tRNA m7G methyltransferase hadaddun sunadaran sunadarai METTL1 da WDR4 sun karu sosai a cikin kyallen ESCC kuma sun ba da gudummawa ga rashin hangen nesa na ESCC.Tun daga wannan lokacin, binciken METTL1 da WDR4 da ke da alaƙa da ƙwayar ƙwayar cuta a cikin ƙirar ƙirar ƙirar ESCC sun tabbatar da cewa haɓaka aikin tRNA m7G methyltransferase yana haifar da haɓaka-ka'idojin bayyanar waɗannan sunadaran guda biyu, wanda hakan yana haɓaka haɓakar ƙwayoyin ESCC.Wannan binciken yana nuna muhimmin aikin oncogenic na gyare-gyaren tRNA m7G da ba daidai ba a cikin ESCC kuma yana ba da shawarar cewa niyya METTL1 da hanyoyin siginar sa na ƙasa na iya zama alƙawarin maƙasudin warkewa don maganin ESCC.
Samfurin da aka taimaka:
Mouse Tail Direct PCR Kit Foregene#TP-01333
Asalin mahada:
https://www.nature.com/articles/s41467-022-29125-7
02
Idan: 14.903
An buga ta: Maɓalli na Jiha na Binciken Inganci a Magungunan Sinanci, Cibiyar Nazarin Kiwon Lafiyar Sinawa, Jami'ar Macau
Babban abun ciki:
Tare da haɓaka fasahar roba, mRNA ya zama sabon nau'in magungunan warkewa.Koyaya, samun ingantaccen isar da intracellular na magungunan mRNA ya kasance mai wahala saboda azancin mRNA zuwa RNases.Wannan binciken yana ba da shawarar ingantaccen tsarin isarwa na nanostructures-conjugated polyphenol wanda zai iya isar da IL-10 mRNA yadda ya kamata a cikin vivo.Bayan yin amfani da wannan nanostructure don isar da magungunan mRNA, matakan IL-10 sun kasance musamman da aka tsara su, suna hana bayyanar cututtuka masu kumburi, inganta gyaran gyare-gyare na mucosal, kare kwayoyin epithelial na colonic daga apoptosis, da kuma hana dextran sodium sulfate-induced m da na kullum ƙananan ƙwayar murine colitis yana da mahimmancin ƙira don maganin cututtukan ƙwayar cuta.
Samfurin da aka taimaka:
Jimlar Dabbobin Keɓewar RNA Kit Foregene#RE-03011
Asalin mahada:
03
Idan: 12.499
An buga ta: Laboratory of Experimental Animal Disease Model, Kwalejin likitancin dabbobi, Jami'ar aikin gona ta Sichuan
Babban abun ciki:
Damuwar rashin haihuwa na maniyyi shine babban abin da ke haifar da rashin haihuwa na namiji, don haka zaɓi ne mai yiwuwa kuma mai tasiri don kafa nau'in dabbar rashin haihuwa na namiji ta hanyar daidaita tsarin antioxidant a cikin hanyar da aka yi niyya.Duk da haka, ƙwayoyin ƙwayoyin ƙwayoyin cuta na musamman na antioxidant ba a bayyana su ba.Wannan binciken ya tabbatar da cewa LanCL1 gene wani takamaiman maganin antioxidant ne a cikin ƙwayoyin ƙwayoyin cuta na namiji, kuma an rage bayyanarsa ko kuma yana hade da rashin haihuwa na namiji.Mice na mice a cikin kwayar LanCL1 suna nuna lalacewar maniyyi da rashin haihuwa.A cikin aiwatar da bambance-bambancen maniyyi na ƙwayoyin ƙwayoyin cuta, da zarar yanayin iskar oxygen ya faru, ƙayyadaddun furotin na musamman na 1 (SP1) za su daidaita maganganun LanCL1, ta haka yana hana tsarin iskar oxygenation na maniyyi.Wannan binciken ya nuna cewa hanyar SP1-LanCL1 tana taka muhimmiyar rawa wajen daidaita tsarin homeostasis na testicular da kuma haihuwa na namiji wanda aka daidaita ta hanyar redox balance, kuma yana nuna cewa LanCL1-gyaran mice suna da aikace-aikace masu ban sha'awa a matsayin samfurin dabba na rashin haihuwa.
Samfurin Talla:
Mouse Tail DNA Mini Kit Foregene#DE-05211
Asalin mahada:
http://europepmc.org/article/MED/35469022
◮Ba a buƙatar tsarkakewar DNA mai cin lokaci da tsada.
◮ Bukatar samfurin kadan ne, kawai a dauki iri daya.
◮Ba a buƙatar magani na musamman kamar niƙa da niƙa, kuma aikin yana da sauƙi.
◮Ingantacciyar tsarin PCR yana bawa PCR damar samun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙarfi da haƙuri ga masu hanawa PCR.
◮Babu buƙatar damuwa game da lalacewar RNA.Duk tsarin ba shi da RNase-Free.
◮ Cire DNA da kyau ta amfani da Rukunin Tsabtace DNA.
◮ Cire DNA ba tare da ƙara DNA ba.
◮ Mai Sauƙi—dukkan ayyuka ana kammala su a cikin zafin daki.
◮Ayi sauri- ana iya kammala aikin a cikin mintuna 30.
◮Safe-ba a yi amfani da reagent Organic ba.
◮Maɗaukakin tsarki-OD260/280≈1.8-2.1.
◮Babu gurɓatar RNase:Rukunin DNA-Only da kit ɗin ya samar yana ba da damar cire RNA daga DNA ɗin kwayoyin halitta ba tare da ƙara RNase ba yayin gwajin, yana hana dakin gwaje-gwaje daga kamuwa da RNase na waje.
◮Gudun sauri:Foregene Protease yana da ayyuka mafi girma fiye da irin wannan proteases, kuma yana narkar da samfuran nama da sauri;aikin yana da sauƙi, kuma ana iya kammala aikin hakar DNA na genomic a cikin mintuna 20-80.
◮Dace:Ana yin centrifugation a dakin da zafin jiki, kuma babu buƙatar ƙananan zafin jiki na 4 ° C ko hazo na ethanol na DNA.
◮Tsaro:Ba a buƙatar hakar reagents na halitta.
◮Babban inganci:DNA ɗin da aka fitar yana da manyan gutsuttsura, babu RNA, babu RNase, da ƙarancin ion abun ciki, wanda zai iya biyan buƙatun gwaje-gwaje daban-daban.
◮Tsarin Micro-elution:Yana iya ƙara ƙaddamar da DNA na genomic, wanda ya dace don ganowa ko gwaji.
Lokacin aikawa: Satumba-13-2022
