Fage
A cikin 'yan shekarun nan, ƙananan ƙwayoyin cuta (EVs) sun jawo hankalin mutane a matsayin kayan aiki mai mahimmanci na warkewa;duk da haka, ba a ba da rahoton tasirin magani na EVs akan endometriosis ba.Endometriosis cuta ce ta gama gari wacce ba ta da kyau wacce ke shafar 10-15% na matan da suka kai shekarun haihuwa, yana haifar da alamu iri-iri, yana haifar da raguwar ingancin rayuwa da kuma nauyi mai girma na zamantakewa.
Gabatarwa Labari
A ranar 20 ga Yuli, 2021, ƙungiyar bincike ta Farfesa Wang Guoyun daga Asibitin Qilu na Jami'ar Shandong ta buga wata takarda bincike mai suna "M1 Macrophage-Derived Nanovesicles Repolarize M2 Macrophages for Hana Ci gaban Endometriosis" akan Frontiers a cikin rigakafi, wanda yayi magana akan samuwar M1 macrophages.Yiwuwar nanovesicles (NVs) a cikin maganin endometriosis.
Wannan labarin yana amfani da hanyar ci gaba da extrusion don shirya M1NVs, kuma yana amfani da hanyar al'adu don nazarin canje-canje a cikin angiogenesis, ƙaura, mamayewa da sauran alamun eutopic endometrial stromal sel (EM-ESCs) daga marasa lafiya tare da endometriosis.A lokaci guda, an kafa samfurin linzamin kwamfuta na endometriosis, kuma an yi wa mice magani tare da PBS, MONVs ko M1NVs, bi da bi, don kimanta inganci da amincin M1NV a cikin maganin endometriosis.
Sakamakon ya nuna cewa in vitro M1NVs na iya kai tsaye ko a kaikaice hana ƙaura da mamayewar EM-ESCs, da hana angiogenesis.A cikin ƙirar linzamin kwamfuta, M1NVs suna hana faruwar endometriosis ta hanyar M2 macrophage reprogramming ba tare da haifar da lalacewar gabobin ba.Ya nuna cewa M1NVs na iya hana faruwar endometriosis kai tsaye, kuma ana iya hana su ta hanyar canza nau'in macrophages na M2 zuwa nau'in M1.Saboda haka, amfani da M1NV na iya zama sabuwar hanya don maganin endometriosis.
Taimakon Fargaba
A cikin binciken, saboda an shirya M1NV ta hanyar ci gaba da matsawa M1 macrophages, labarin ya yi amfani da qRT-PCR don gano abubuwan da ke haifar da kumburi da alamun M1 macrophage iNOS, TNF-a da IL-6 mRNA a cikin M1NV da M1 macrophages.Canje-canje masu yawa na dangi.Sakamakon ya nuna cewa M1NVs sun ƙunshi ƙarin abubuwan da ke haifar da kumburi mRNA da alamun macrophage M1, wanda ke nuna cewa M1NVs na iya riƙe halayen aiki na ƙwayoyin M1 yadda ya kamata.Wannan hanyar bincike tana amfani da QuickEasy Cell Direct RT-qPCR Kit-SYBR Green I na Foregene
Cell Direct RT-qPCR Kitcikakkun bayanai

Yanayin aikace-aikacen:
 
1. Tsarin tsararrun kwayoyin halitta da bincike na magana, tabbatar da yawan wuce gona da iri ko tasirin tsangwama, gwajin magani, da dai sauransu;
2. Gano maganganun kwayoyin halitta na ƙwayoyin da ke da wuyar noma irin su sel na farko, ƙwayoyin sel, da ƙwayoyin jijiya;
3. Gano mRNA a cikin samfurori irin su exosomes da nanovesicles.
Siffofin: