Menene DirectPCR?
Fasahar DirectPCR tana nufin cewa ba tare da rabuwa da tsarkakewar kwayoyin acid nucleic (ciki har da DNA da RNA) na samfurori na nama daban-daban ba, kawai nama da tsarin tantanin halitta sun lalace ta hanyar lysis na protease, an saki nucleic acid a cikin maganin lysis, kuma an ƙara maganin lysis kai tsaye.Tsarin amsawa na PCR fasaha ce don haɓaka kwayar halittar da aka yi niyya.
Neman canji
Rabewa da hakar al'amuran acid nucleic
Tun da aka kirkiro fasahar PCR shekaru 30 da suka gabata, masu bincike sun damu da rabuwa da kuma fitar da sinadarin nucleic acid.Amfani da samfuran nama don aiwatar da halayen PCR kai tsaye shine mafarkin masu bincike da yawa.Amma wannan mafarkin bai cika shekaru 30 ba.Dalilin shi ne cewa nama mai tarwatsewa zai saki yawancin abubuwan hanawa.
Wadannan abubuwan hanawa za su sami tasirin hanawa mai ƙarfi akan halayen PCR, wasu za su haifar da firam ɗin da samfuri ba za su iya ɗaure ba, wasu suna da aikin denaturation mai ƙarfi na furotin, wanda ke haifar da rashin kunnawa na polymerase na nucleic acid, kuma wasu kai tsaye suna hana firam da samfuri daga ɗaure .Waɗannan su ne duk abubuwan da ke haifar da amsawar PCR ba ta ci gaba da kyau ba.
A al'adance, lokaci-lokaci mutane suna amfani da na'urorin haɓaka PCR don haɓaka hankali, ƙayyadaddun ƙayyadaddun da haɓaka haɓakar halayen PCR.Duk da haka, saboda daban-daban masu hana PCR da ke kunshe a cikin samfurori na nucleic acid daga tushe daban-daban, yin amfani da dogon lokaci na yawan masu haɓakawa a fili ba shi da daraja asara, farashin yana da yawa, kuma aikin yana da wahala.
Farashin DirectPCR
Babban ci gaban duniya --Techniques Biyu
Foregene ya sami ci gaba a wannan fanni na fasaha a duniya.Fasahar Foregene DirectPCR tana da maki na fasaha guda biyu, waɗanda ke baiwa Foregene damar zama majagaba kuma jagoran fasahar DirectPCR.
Na farko, hanyar gyare-gyaren nucleic acid polymerase mai haƙƙin mallaka.Forgene yana da hanyar gyare-gyaren nuclease da aka ƙirƙira, wanda aka yi niyya a yankin daure don ƙara ƙarfin nuclease da samfuri, tare da babban aiki, ingantaccen haɓakawa da kwanciyar hankali na thermal.
Na biyu, dabarar PCR Mix da aka inganta don nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan haɓaka halayen haɓaka da haɓaka haɓaka haɓaka haɓaka haɓaka haɓaka haɓaka haɓaka haɓaka haɓaka haɓaka haɓakar juriya na polymerase ga masu hana PCR da tabbatar da ingancin haɓakawa.
Daidai saboda mahimman ci gaban fasaha guda biyu da aka ambata a sama shine Foregene ya gane ainihin DirectPCR akan sikelin duniya.Ko kyallen dabba ne na kowa, ruwan jiki, kyallen shuka, ganye ko tushen tukwici, ko ma iri iri, masu amfani za su iya samun sauƙin haɓaka PCR kai tsaye ba tare da wani fashewar inji ko tsarin tsarkakewa na nucleic acid ba.
Don na'urorin DirectPCR na jerin tsire-tsire da jerin dabbobi waɗanda aka ƙaddamar, Foregene na iya yin alfahari da cewa muna da manyan alamun wasan kwaikwayon duniya.A nan gaba, Foregene zai ci gaba da ƙaddamar da samfurori na samfurori waɗanda har yanzu suna da alamun jagorancin duniya (ko ma na musamman).
Don ƙarin bayani, shigar:
http://www.foregene.com/http://www.foreivd.com/
Lokacin aikawa: Fabrairu-27-2017
