Haihuwar PCR
PCR (Polymerase Chain Reaction)
Sama da shekaru 30 ke nan tun da ƙirƙirar sarkar polymerase.Fiye da shekaru 30, bayan masana da yawa a duniya sun ci gaba da haɓakawa da haɓakawa, fasahar PCR ta zama mafi yadu kuma akai-akai amfani da ita kuma mafi mahimmancin hanyar bincike na asali a duk fagen Kimiyyar Rayuwa.
The TouchDown PCR, Real-Time PCR, Multi PCR, da dai sauransu ci gaba a kan tushen da fadi da aikace-aikace na gargajiya PCR fasaha, kazalika da sabuwar fito da Digital PCR (digital PCR), sun ƙwarai wadãtar da bincike hanyoyin da mafi yawan na kimiyya masu bincike da kuma ƙwarai hanzarta ci gaban tsarin na zamani Life Sciences, musamman kwayoyin halitta, ya ba da babbar gudumawa na bil'adama a matsayin dukan rayuwa da yanayi.
Lalacewar fasahar PCR ta gargajiya
Complex nucleic acid rabuwa dahakar:
★ Fasahar PCR ta al'ada: ake buƙata
★ PCR da aka samu fasaha: ake buƙata
Samfuran DNA da RNA: manyan bambance-bambance, buƙatun aiki masu wahala
★ Hatsarin Jiki: Reagents masu guba suna cutar da jiki
Fasahar PCR ta al'ada da fasaha na asali suna da buƙatu-rabuwar acid nucleic da tsarkakewa.
Duk wani samfurin halitta yana buƙatar shiga cikin jerin sarƙaƙƙiya da sarrafa samfur don samun samfuran acid nucleic waɗanda suka dace da buƙatun fasahar PCR.
Rabuwa da hakar DNA da RNA ya kasance koyaushe babban aiki ne wanda masu binciken kimiyya masu dacewa ke buƙatar maimaita kowace rana.
Saboda ɗimbin bambance-bambancen da ke tsakanin samfuran, rarrabuwar kawuna da tsarin cirewar DNA da RNA su ma sun bambanta sosai.Wannan aikin yana buƙatar babban matakin ƙwarewar fasaha don masu aiki.Dabarun rarrabuwa da haɓakar al'ada suna buƙatar tuntuɓar dogon lokaci tare da wasu magunguna masu guba masu guba.Zai haifar da lalacewar da ba za a iya jurewa ba ga jikin ma'aikacin, har ma ya haifar da lalacewa kai tsaye yayin gwajin.
A lokaci guda kuma, ga waɗanda ke da adadi mai yawa na samfurori don yin nazari, rabuwa da cirewar acid nucleic aiki ne mai wahala.
Keɓewar Nucleic acid da kayan aikin hakar a kasuwa yanzu sun balaga kuma akwai samfuran da yawa, amma kusan iri ɗaya ne.Ko silica gel membrane ginshiƙin centrifugal kit ko kayan aikin ƙirar ƙirar maganadisu, yana ɗaukar lokaci mai yawa kuma yana da tsada.Baya ga farashin kit ɗin, akwai kuma buƙatu na musamman don kayan aikin dakin gwaje-gwaje.Wurin aiki mai sarrafa kansa da aka yi amfani da shi a cikin hanyar ƙwanƙwasa maganadisu wani babban kayan aiki ne mai ƙima, wanda babban kuɗi ne ga dakin gwaje-gwaje.
a takaice
Kafin gudanar da gwaje-gwaje na PCR, pretreatment na samfurori shine makawa kuma ko da yaushe ciwon kai ga masu bincike.Yadda za a magance wannan matsala da kuma ko za a iya yin gwaje-gwaje na PCR ba tare da rarrabuwa da cirewar acid nucleic ba ya kasance tunanin yawancin masu binciken kimiyya da ma'aikatan dakin gwaje-gwaje na asibiti.
Maganin Foregene
Bayan shekaru na bincike mai ɗorewa kan fasahar PCR kai tsaye da kayan aikin da ke da alaƙa, Forgene ya sami nasarar keta ƙulla da dama kuma ya sami nasarar cimma PCR kai tsaye don nau'ikan samfuran daban-daban tare da juriya mai ƙarfi da daidaitawa, yana ba masu bincike damar kawar da ɓarna mai haɗari da haɗari da haɓakar acid nucleic.Wannan zai rage yawan ƙarfin aikin kowa, da hanzarta aikin gwaji, da kuma adana binciken kimiyya da tsadar gwaji.
Fahimtar Forgene da sanin DirectPCR
Na farko, fasahar DirectPCR fasaha ce ta PCR kai tsaye don nau'ikan samfuran samfuran halitta daban-daban.A ƙarƙashin wannan yanayin fasaha, babu buƙatar rabuwa da cire acid nucleic, kuma ana amfani da samfurin nama kai tsaye a matsayin abu, kuma ana ƙara maƙasudin kwayoyin halitta don amsawar PCR.
Na biyu, fasahar DirectPCR ba fasahar haɓaka samfurin DNA ta gargajiya ce kawai ba, har ma ta haɗa da samfurin RNA na juyar da PCR.
Na uku, fasahar DirectPCR ba wai kai tsaye tana aiwatar da halayen PCR na yau da kullun akan samfuran nama ba, har ma ya haɗa da halayen qPCR na Real-Time, wanda ke buƙatar tsarin amsawa don samun ƙarfi mai ƙarfi don tsayayya da tsangwama a bango da kuma adawa da masu kashe hasken wuta.
Na hudu, samfuran nama da aka yi niyya da fasahar DirectPCR kawai suna buƙatar sakin samfuran nucleic acid kuma kada su cire sunadaran, polysaccharides, ions gishiri, da sauransu waɗanda ke tsoma baki tare da amsawar PCR.Wannan yana buƙatar polymerase na nucleic acid da PCR Mix a cikin tsarin amsawa don samun ingantacciyar anti-reversibility da daidaitawa, kuma yana iya tabbatar da aikin enzyme da daidaiton kwafi a ƙarƙashin yanayi masu rikitarwa.
Na biyar, samfuran nama da aka yi niyya da fasahar DirectPCR ba a yi su da wani magani na haɓaka acid na nucleic ba, kuma adadin samfurin ya yi ƙanƙanta, wanda ke buƙatar kulawa mai girma da haɓaka haɓakar tsarin amsawa.
Kammalawa
Fasahar DirectPCR tana ɗaya daga cikin mahimman ci gaban fasaha da sabbin abubuwa a cikin shekaru 30 da suka gabata tun lokacin haifuwar fasahar PCR.Forgene yana da kuma zai ci gaba da kasancewa majagaba kuma mai haɓaka wannan fasaha.
Hasashen aikace-aikacen fasahar DirectPCR yana da faɗi sosai.Ci gaba da haɓakawa da haɓaka wannan fasaha tabbas zai kawo sauye-sauye na sauye-sauye ga binciken kimiyya da aikin dubawa.Wannan juyi ne na fasaha na PCR.
Lokacin aikawa: Fabrairu-21-2017
