W:Dan'uwa, an kammala rubutun baya, kuma a ƙarshe mataki na ƙarshe - PCR mai kyalli!Hey, ɗan'uwa, menene PCR na ainihin-lokaci?
M:PCR koyaushe kuna sani, daidai?
W: Ka sani, PCR shine maganin sarkar polymerase, kuma fasaha ce da ke ƙara DNA!
M: Ya kusan… Hanya ce ta fasaha don haɓaka jerin abubuwan da aka yi niyya zuwa fiye da sau miliyan ɗaya ta hanyar ƙayyadaddun ƙayyadaddun abubuwa, amma wannan kawai yana nuna kasancewar ko rashi na jerin abubuwan da ake niyya, kuma ba zai iya tantance adadin samfurin farko ba.Fluorescence quantitative PCR hanya ce ta fasaha da aka haɓaka don magance wannan matsalar.PCR na ainihi da adadin PCR (qPCR) gabaɗaya suna nuni da shi.
W: Bambancin da PCR na yau da kullun ya ta'allaka ne a cikin ƙididdige ƙimar haske?
M: Ee, wato, a cikin tsawaita mataki na kowane zagayowar PCR, ana gano ƙarfin siginar kyalli, kuma ana iya ƙididdige adadin samfurin farko bisa ga canjin ƙarfin siginar.
W: Yana jin rikitarwa sosai!Shin dole ne in lissafta wannan da kaina?
M: Wannan yana buƙatar ku koya a hankali, yana da kyau ku iya fahimtar ƙa'idar.Yanzu zaku iya mayar da hankali kan ƙimar CT da farko, wanda shine ingantattun bayanan gwaji, kuma girmansa yayi daidai da girman adadin samfurin farko.Akwai kuma zane-zane irin su na'urorin haɓakawa da narkewar lanƙwasa.Suna da hujja ta ƙarshe kan ko gwajin ku ya yi nasara ko a'a.
W: Oh, ɗan'uwa.Sannan zan yi gwajin qPCR!
M: Kula da hankali na musamman don kada ku ƙara samfuri zuwa yankin reagent, in ba haka ba za'a iya gurɓata haɗin da ba a yi amfani da ku ba!
W: samu!

Real Time PCR Easyᵀᴹ-SYBR Green I Kit

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