Hakar acid nucleic fasaha ce ta gabatarwa don gwaje-gwajen ilimin halitta.Ko zai iya ƙware da amfani da wannan fasaha sosai kuma ya zama buƙatu ga masu gwaji daga baya don haɓakawa zuwa masanan gwaji.
Dangane da kayan bincike daban-daban, an raba masu gwaji zuwa nau'ikan nau'ikan guda uku masu zuwa:
Manyan dabbobi, Manyan tsirrai, Manyan ƙwayoyin cuta
Kowane nau'in mai gwaji zai ci gaba da kwafi kwafi don haɓaka saitin ƙwarewa na keɓance don takamaiman samfura.
A yau, editan yana so ya kawo wasu kayan aiki da kayan aiki waɗanda ba su samuwa ta cikin gidan kurkuku ga masu gwaji.Tare da su, zaku iya haɓaka haɓakawa da sauri a duk kwatance.Ba mafarki ba ne don zama allahn gwaji.
Wannan batu na ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun ƙwararrun masana'antu ne (masu gwadawa na sauran sana'o'i kuma za su iya koyo daga gare ta ~).
Na yi imani cewa kowane ƙarni na masu gwaji za su ji labarin hanyoyin hako acid nucleic na tsire-tsire waɗanda suka shuɗe daga tsara zuwa tsara daga magabata.Manyan sunaye guda uku a cikin masana'antar hakar tsirrai.
Ko da yake manyan mutane suna da kyau, har yanzu suna da wasu gazawar da ba za a iya kaucewa ba.
Hanyar TRIzol
Sau da yawa ana amfani dashi don cirewa
RNA
●Mai guba da taurin kai
●Dauke da gurbacewar kwayoyin halitta
Hanyar CTAB
Sau da yawa ana amfani dashi don cirewa
kwayoyin halittaDNA
● Cin lokaci
●Mafi wuya a cire CTAB ta hanyar wankewa fiye da gishiri, ƙaramin adadin ragowar zai yi tasiri sosai akan aikin enzyme.
Hanyar SDS
Sau da yawa ana amfani dashi don cirewa
plasmid
●Ba dace da shuke-shuke da babban abun ciki na polysaccharide
●Lalacewar sunadaran
A cikin wannan al'umma ta zamani tare da sabuntawar sauri sau biyu, hanyoyin aiki na gargajiya ba su iya biyan bukatun masu gwaji ba, kuma haɓaka kayan aiki yana nan kusa.
An gwada Kit ɗin Haɓakar Acid na Foregene N sau da yawa ta masu gwaji.Yana da sauƙi don aiki, baya amfani da duk wani reagents mai guba, kuma baya ƙara kowane enzymes.Acid nucleic da aka samu yana da tsafta mai yawa, wanda ke da mahimmanci don aikin hakar yau da kullun na masu gwaji masu kyau.
Foregene RNA Isolation kit
Anan, zan kuma bayyana wata dabarar aiki ta ɓoye ga masu gwajin, wanda shine zaɓar hanyar da ta dace da hakowa bisa ga nau'in shuka (hanyar tantance nau'in shuka shine a ƙarshen labarin!), Domin wasu tsire-tsire na polysaccharides da polyphenols suna da yawa a cikin abun ciki, kuma hanyoyin gargajiya suna da mutuwa.Bai isa sarrafawa ba.
To, kamar haka...
Hanyar TRIzol kai tsaye ta durƙusa a ƙarƙashin samfurori tare da manyan matakan polysaccharides da polyphenols kamar ganyen ayaba, ganyen ginkgo da ganyen auduga.
Don wannan dalili, Foregene ya haɓaka kayan aiki masu inganci guda biyu don masu gwaji.Kayan cirewar RNA na shuka tare da ƙananan polysaccharide da polyphenol da babban polysaccharide da polyphenol na iya kashe abokan gaba ta hanyar da aka yi niyya, ta yadda masu gwajin ba su ƙara jin tsoron tsire-tsire daban-daban.Kawai canza kayan aiki kai tsaye!
Baya ga buƙatun haɓaka hanyar hakar, ana buƙatar haɓaka samfuran da ake aiwatarwa kafin aiwatarwa.
Saboda tsari na musamman na samfuran tsire-tsire, saiwoyin da mafi ƙarfi na wasu tsire-tsire sun fi wuya a niƙa da murkushe su daidai.
A cikin masana'antar niƙa, kusan kashi 80% na mutane suna amfani da ruwa na nitrogen don niƙa, yayin da ƙasa da kashi 20% ke amfani da injin niƙa na lantarki da niƙa ƙwanƙwasa.
Kodayake nika nitrogen mai ruwa yana da arha kuma mai sauƙin amfani, abin tsoro ne ga masu gwaji tare da babban buƙatar samfurin don niƙa kowane lokaci da ƙoƙari!Idan ba'a tsaftace turmi ba, zai haifar da gurɓata tsakanin samfurori.
Tasirin niƙa na lantarki ya bambanta daga mutum zuwa mutum, kuma yanayin aiki na masu gwaji zai yi tasiri sosai akan amincin nucleic acid, kuma ana buƙatar aiki mai zafi idan ya cancanta.
don haka, niƙa dutsen dutse oscillation za a iya daukarsa a matsayin na yanzu sauki da kuma tasiri nika kayan aiki.
Hanyoyin juzu'i na gama-gari sun haɗa da juzu'i a tsaye, jujjuyawar jujjuyawar juzu'i da motsi mai girma uku (hanzali).Koyaya, Foregene's tissue crusher ya karya ta samfurin da ke akwai kuma ya ɗauki sabuwar hanyar oscillation ta 4D don sanya niƙa ta zama iri ɗaya.
Haɗu da buƙatun samfurin daban-daban, mai sauƙin aiki
Fasahar murkushewa ta musamman ta 4D, ingantaccen aikin murkushe yana ƙaruwa da 20%
Samfurin an rufe shi gaba ɗaya, babu gurɓataccen giciye
Natsu, ≤56 decibels
Kwatanta sakamakon gwajin da igiyar ruwa~
Gwajin samfurin: 50mg ganyen alkama (ƙananan abun ciki na polysaccharides da polyphenols)
Cire nau'in acid nucleic: RNA
Adadin wuri: 5 μl
Kayan aikin hakar da aka yi amfani da shi shine Foregene Plant Total RNA Extraction Kit (RE-05011)
Idan aka kwatanta bayanai da jadawali na electrophoresis, za a iya ganin cewa taro da tsarkin RNA da aka fitar bayan na'urar niƙa ya fi na niƙan ruwa nitrogen.A lokaci guda, saboda niƙa na'ura na iya karya samfurin gaba ɗaya, ƙananan RNA za a iya fitar da su da kyau kuma ana iya kiyaye amincin RNA.
Samfurin gwaji: 50mg ginkgo ganye (high polysaccharide da polyphenol abun ciki)
Cire nau'in acid nucleic: RNA
Adadin wuri: 5 μl
Amfani da Foregene Polysaccharide Polyphenol Shuka Total RNA Hakar Kit (RE-05021)
Dubi wannan rukunin samfuran tare da babban abun ciki na polysaccharides da polyphenols.Tasirin ya ma fi fitowa fili.Tare da tsafta iri ɗaya, ƙaddamar da injin niƙa RNA ya kusan sau 5 na niƙa nitrogen, kuma ƙaramar RNA har yanzu ba a ganuwa ~
Idan kun ji cewa babu ɗayan kayan aikin da ke sama da sauri isa, to dole ne editan ya fito da kayan aiki na ƙarshe!
Plant Direct PCR jerin
Babban fasalin wannan kayan aiki shine cewa ganyen shuka ko tsaba basa buƙatar ƙasa da karye, kuma ana jefa su kai tsaye cikin bututun EP don lysis, kuma ana iya amfani da cakuda lysis da aka samu azaman samfuri don amsawar PCR.Yana da sauƙi kamar mataki ɗaya da sauri!A lokaci guda kuma, tsarin PCR kai tsaye na Foregene Plants suna sanye da tsarin hana gurɓataccen gurɓataccen iska na UNG, ta yadda masu gwajin ba za su ƙara damuwa da gurɓataccen iska a cikin dakin gwaje-gwaje ba kuma za su iya yin gwajin cikin sauƙi.
Shawarwari na hukunci:
Yadda za a yi hukunci ko itace polysaccharide polyphenol shuka
Ɗauki kusan 20mg na nama toshe kuma saka shi a cikin bututu mai tsabta na 1.5ml, ƙara 200ul 200mM NaOH bayani, sanya shi a 95 ℃ na 10min, lura da canjin launi bayan lysis.
1. Idan launi na lysate kore ne ko rawaya mai haske, yana nufin cewa abun ciki na polyphenols nama yana da ƙasa, kuma ana bada shawarar yin amfani da jerin Foregene Plant.
2. Idan lysate yana da launin ruwan kasa-rawaya ko launin ruwan kasa-ja, yana nufin cewa abun ciki na polyphenol na nama yana da yawa, kuma ana ba da shawarar yin amfani da Foregene Polysaccharide Polyphenol Plant Plus Series.
Lokacin aikawa: Agusta-20-2021
