Na yi imani cewa kowa da kowa zai fuskanci irin waɗannan matsalolin ko kuma irin waɗannan matsalolin yayin yin halayen PCR, amma yawancin su ana iya rarraba su zuwa manyan matsaloli guda biyu:

Ƙwaƙwalwar ƙirar halitta (ƙarfafawa);
Yawancin haɓakar kwayoyin halitta marasa manufa.
Amfani da additives yana ɗaya daga cikin dabarun gama gari don magance waɗannan matsalolin.Yawanci matsayin additives yana da bangarori biyu:
tsarin sakandarena kwayoyin halitta (tsari na biyu);
Rage ƙayyadaddun ƙira.
A yau, editan zai gabatar muku a taƙaice abubuwan da aka haɗa a cikin halayen PCR da ayyukansu.
Additives da ke rage tsarin na biyu
sulfoxide(DMSO)
samfurin kwayoyin halittatare da babban abun ciki na GC.Koyaya, DMSO shima yana rage ayyukan Taq polymerase sosai.Sabili da haka, kowa ya kamata ya daidaita samfurin samfurin da kuma aikin polymerase.Editan yana ba da shawarar cewa zaku iya gwada ƙididdiga daban-daban na DSMO, kamar daga 2% zuwa 10%, don nemo maida hankali wanda ya dace da gwajin ku.
Abubuwan wanke-wanke marasa ionic
Abubuwan wanka marasa ionic, kamar 0.1-1% Triton X-100, Tween 20 ko NP-40, yawanci suna rage tsarin na biyu na DNA.Ko da yake wannan na iya ƙara haɓakawa na samfurin samfuri, zai kuma haifar da matsala na ƙarawa da ba takamaiman ba.Don haka, waɗannan abubuwan ƙari suna aiki da kyau don halayen PCR masu ƙarancin amfanin ƙasa ba tare da tarkace ba, amma ba da kyau ga halayen PRC marasa ƙazanta ba.Wani fa'idar abubuwan wanke-wanke marasa ionic shine rage gurɓataccen SDS.Yawancin lokaci a lokacin aikin hakar DNA, za a kawo SDS zuwa mataki na PCR, wanda ya hana aikin polymerase sosai.Sabili da haka, ƙara 0.5% Tween-20 ko Tween-40 zuwa amsawar zai iya kawar da mummunan tasirin SDS.
Betaine_
Betaine na iya inganta haɓaka DNA ta hanyar rage haɓakar tsarin sakandare kuma gabaɗaya shine “asiri” ƙari ga kayan PCR na kasuwanci.Idan kuna son amfani da betaine, yakamata ku sanya betaine ko betaine mono-hydrate (Betaine ko Betaine mono-hydrate), amma ba betaine hydrochloride (Betaine HCl), daidaita zuwa matakin ƙarshe na 1-1.7M.Betaine kuma na iya taimakawa inganta ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai saboda yana kawar da dogaron abun da ke ciki na tushe na narkewar DNA/ denaturation DNA.
Additives don rage ƙayyadaddun priming
Formamide
Formamide abu ne da aka saba amfani da shi na kwayoyin PCR.Yana iya haɗuwa tare da babban tsagi da ƙaramin tsagi a cikin DNA, ta haka yana rage kwanciyar hankali na master DNA helix biyu da rage zafin narkewar DNA.Matsakaicin foramide da aka yi amfani da shi a cikin gwaje-gwajen PCR yawanci shine 1% -5%.
Tetramethylammonium chloride( TMAC)
Tetramethylammonium chloride na iya ƙara ƙayyadaddun ƙayyadaddun haɓakawa (ƙayyadaddun ƙayyadaddun haɓakawa) da haɓaka zafin narkewar DNA.Don haka, TMAC na iya cire ƙayyadaddun firam ɗin da ba takamaiman ba kuma ya rage ɓarna DNA da RNA.Idan kuna amfanimasu lalacewaa cikin amsawar PCR, tuna don ƙara TMAC, wanda aka saba amfani dashi a taro na 15-100mM.
Sauran Abubuwan Haɗin Kan Jama'a
Baya ga nau'o'in additives guda biyu da aka ambata a sama, akwai abubuwa da yawa na gama gari a cikin halayen PCR, kodayake suna da ayyuka daban-daban, suna da mahimmanci.
Magnesium ion
Magnesium ion shine madaidaicin cofactor (cofactor) na polymerase, wato, ba tare da ion magnesium ba, polymerase baya aiki.Duk da haka, yawancin ions na magnesium kuma na iya rinjayar yadda ya dace na polymerase.Matsakaicin ions na magnesium a cikin kowane halayen PCR zai bambanta.Ma'aikatan chelating (irin su EDTA ko citrate), ƙaddamar da dNTPs da sunadaran sunadaran duk suna shafar ƙaddamar da ions na magnesium.Don haka, idan kuna da matsaloli tare da gwajin PCR ɗinku, zaku iya ƙoƙarin canza ma'aunin ion magnesium daban-daban, misali, daga 1.0 zuwa 4.0mM, tare da tazara na 0.5-1mM tsakanin.
Ya kamata a lura da cewa daskarewa-narke hawan keke da yawa na iya haifar da ƙaddamar da hankali na maganin magnesium chloride.Don haka, dole ne a narkar da shi gaba ɗaya kafin kowane amfani, kuma a haɗa shi da kyau kafin amfani da shi.
Serum albumin(Bovine albumin, BSA)
A cikin gwaje-gwajen sunadarai na kwayoyin sunadarai, Bugum Album na da kowa ya zama cike da abinci mai cike da abinci, musamman ma a taƙaicewar enzyme narkewa da gwaje-gwajen Enzyme.A cikin halayen PCR, BSA yana taimakawa wajen rage gurɓatattun abubuwa kamar mahaɗan phenolic.Sannan kuma an ce yana iya rage mannewar da ake yi a bangon bututun gwajin.A cikin amsawar PCR, yawanci adadin BSA da aka ƙara zai iya kaiwa 0.8 mg/ml.
 
Samfura masu dangantaka:
Jarumi PCR(da rini)
Jarumi PCR