salam, abokai!
Bayan yin hakar acid nucleic na tsawon shekaru masu yawa, kowa yana sane da gaskiyar cewa ingancin hakar nucleic acid shine mabuɗin nasara da daidaito na gwaje-gwaje na gaba.
Sau da yawa, muna kula kawai ko rabon OD260/OD280 yana cikin kewayon mafi kyau.Kamar haka…
Kamar yadda ake cewa, ba za ku iya yin magana da yawa ba, kuma ba za ku iya yin farin ciki da wuri ba.
Da farko ka saurari Edita ya ba ku ɗan labari!
Irin waɗannan kyawawan tatsuniyoyi sau da yawa suna faruwa a cikin dakunan gwaje-gwaje waɗanda ke fitar da sinadarin nucleic acid.
Kun yi tunanin RNA kawai kuka ɗauka, Yi haƙuri!
A gaskiya, kawai don ba ku san cewa Mista Protein yana ɓoyewa a asirce ba.
Kuna tsammanin kuna son sanya Mista Protein ya bayyana tare da OD260/OD280 guda ɗaya kawai?
Ya yi matashi!Yayi sauki!
Bari mu yi amfani da mafi mahimmancin hanyar tabon azurfa don taimaka muku ganin sunadaran da ke ɓoye a cikin RNA!
| An yi amfani da samfur | OD260/280 | Mkayan aiki mai sauƙi |
| Foregene | 2.09 | Thermo ScientificTMNanoDropTMDaya Ultra-trace UV-Vis Spectrophotometer |
| Trizol | 1.91 |
Tun da dadewa, furotin da RNA abokai ne na kud da kud.
Mista Protein ya fi jin kunya.
Don haka koyaushe ya kasance RNA mai aiki da fara'a don yin wasa da ita.
Wata rana.
RNA ya gaya wa Mista Protein cewa wasu za su karbe shi, kuma Mista Protein ya yi bakin ciki sosai.
Hakanan RNA ya ƙi yarda da furotin Mr, kuma ya ce: "Yaya game da na ɓoye ku, kuna ɓoye cikin tufafina, kuyi ƙoƙarin kada wasu su gan ku, lafiya?"
Mista Protein ya fashe da murmushi kuma cikin farin ciki ya yarda.
A ranar karɓo, Mista Protein ya ɓoye a cikin tufafin RNA kuma ba a same shi ba.Ya bi wanda aka goyo ya koma gida kuma ya yi rayuwa mai dadi tun daga lokacin.
Kwatanta cirewar RNA da ForegeneCellJimlarKit ɗin Hakar RNA
da Trizol Reagent na Kamfanin T
Za mu iya gani a fili karara a kan layin 4 (Trizol), yayin da layin 3 (Foregene) yana da tsabta.Sakamakon ya tabbatar da cewa ƙazantar furotin RNA da aka fitar ta hanyar Trizol har yanzu sun kasance digo da yawa!Kuma layi na 3 (Foregene) kusan ba shi da gurɓataccen furotin saboda mai gwajin ya yi amfani da kayan cirewar Foregene RNA!(Talla ta zo ba zato ba tsammani)
Karin bayani:
https://www.foreivd.com/cell-total-rna-isolation-kit-product/
Lokacin aikawa: Fabrairu-10-2022
